1. 重组蛋白
  2. Others
  3. MAP1LC3B 蛋白, Human

MAP1LC3B 蛋白, Human

目录号: HY-P70909
COA 产品使用指南

MAP1LC3B 是一种关键的类泛素修饰剂,对于自噬体液泡的形成至关重要,可维持细胞稳态。在线粒体自噬中,它调节线粒体数量,抑制活性氧并确保能量效率。MAP1LC3B 蛋白, Human 是重组的 MAP1LC3B 蛋白,由 E. coli 表达,不带标签。MAP1LC3B 蛋白, Human 全长 125 个氨基酸,分子量约为 ~16.15 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥390 In-stock
10 μg ¥650 In-stock
50 μg ¥1800 In-stock
100 μg ¥3100 In-stock
> 100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

MAP1LC3B is a key ubiquitin-like modifier essential for the formation of autophagosome vacuoles, which maintains cellular homeostasis. In mitophagy, it regulates mitochondrial number, suppresses reactive oxygen species and ensures energy efficiency. MAP1LC3B Protein, Human is the recombinant human-derived MAP1LC3B protein, expressed by E. coli , with tag free. The total length of MAP1LC3B Protein, Human is 125 a.a., with molecular weight of ~16.15 kDa.

研究背景

MAP1LC3B 是一种泛素样修饰剂,在自噬体液泡形成中发挥着关键作用,有助于细胞稳态。它参与线粒体自噬,调节线粒体的数量和质量,防止过量的活性氧产生并确保能量效率。在细胞应激期间,它与 C-18 神经酰胺结合,将自噬溶酶体锚定在线粒体外膜上,促进受损线粒体的消除。与参与吞噬细胞膜伸长的 LC3 不同,MAP1LC3B 所属的 GABARAP/GATE-16 亚家族对于后期自噬体成熟至关重要。MAP1LC3B 通过与 TEX264 相互作用进一步促进初级纤毛发生并协调内质网子结构域重塑为自噬体。为了应对营养应激,它会招募辅助因子 JMY,增强肌动蛋白成核和自噬体生物发生。这种多功能蛋白质与多种伙伴相互作用,包括用于包涵体降解的 SQSTM1、ATG13、MAPK15、BNIP3、KEAP1、PCM1 和 TBC1D5,突出显示其参与多种细胞过程和信号通路。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q9GZQ8 (M1-V125)

基因 ID
蛋白结构
N-term
MAP1LC3B (M1-V125)
Accession # Q9GZQ8
C-term
同用名
Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light cha
氨基酸序列

MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV

分子量

Approximately 16.15 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 2 mM DTT, pH 8.0 .

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

MAP1LC3B Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
MAP1LC3B Protein, Human
目录号:
HY-P70909
需求量: