Drug metabolite synthesis by immobilized human FMO3 and whole cell catalysts

Background: Sufficient Reference Standards of drug metabolites are required in the drug discovery and development process. However, such drug standards are often expensive or not commercially available. Chemical synthesis of Drug Metabolite is often difficulty due to the highly regio- and stereo-chemically demanding. The present work aims to construct stable and efficient biocatalysts for the generation of drug metabolites in vitro.

Result: In this work, using benzydamine as a model drug, two easy-to-perform approaches (whole cell catalysis and Enzyme immobilization) were investigated for the synthesis of FMO3-generated drug metabolites. The whole cell catalysis was carried out by using cell suspensions of E. coli JM109 harboring FMO3 and E. coli BL21 harboring GDH (glucose dehydrogenase), giving 1.2 g/L benzydamine N-oxide within 9 h under the optimized conditions. While for another approach, two HisTrap HP columns respectively carrying His₆-GDH and His₆-FMO3 were connected in series used for the biocatalysis. In this case, 0.47 g/L benzydamine N-oxide was generated within 2.5 h under the optimized conditions. In addition, FMO3 immobilization at the C-terminal (membrane anchor region) significantly improved its enzymatic thermostability by more than 10 times. Moreover, the high efficiency of these two biocatalytic approaches was also confirmed by the N-oxidation of tamoxifen.

Conclusions: The results presented in this work provides new possibilities for the drug-metabolizing enzymes-mediated biocatalysis.

Benzydamine; Enzyme immobilization; Flavin-containing monooxygenase isoform 3; Glucose dehydrogenase; Whole cell catalysis.