Abscisic Acid Mediates Salicylic Acid Induced Chilling Tolerance of Grafted Cucumber by Activating H2O2 Biosynthesis and Accumulation

Grafting is widely applied to enhance the tolerance of some vegetables to biotic and abiotic stress. Salicylic acid (SA) is known to be involved in grafting-induced chilling tolerance in cucumber. Here, we revealed that grafting with pumpkin (Cucurbita moschata, Cm) as a rootstock improved chilling tolerance and increased the accumulation of SA, Abscisic acid (ABA) and hydrogen peroxide (H₂O₂) in grafted cucumber (Cucumis sativus/Cucurbita moschata, Cs/Cm) leaves. Exogenous SA improved the chilling tolerance and increased the accumulation of ABA and H₂O₂ and the mRNA abundances of CBF1, COR47, NCED, and RBOH1. However, 2-aminoindan-2-phosphonic acid (AIP) and L-a-aminooxy-b-phenylpropionic acid (AOPP) (biosynthesis inhibitors of SA) reduced grafting-induced chilling tolerance, as well as the synthesis of ABA and H₂O₂, in cucumber leaves. ABA significantly increased endogenous H₂O₂ production and the resistance to chilling stress, as proven by the lower electrolyte leakage (EL) and chilling injury index (CI). However, application of the ABA biosynthesis inhibitors sodium tungstate (Na₂WO₄) and fluridone (Flu) abolished grafting or SA-induced H₂O₂ accumulation and chilling tolerance. SA-induced plant response to chilling stress was also eliminated by N,N'-dimethylthiourea (DMTU, an H₂O₂ scavenger). In addition, ABA-induced chilling tolerance was attenuated by DMTU and diphenyleneiodonium (DPI, an H₂O₂ inhibitor) chloride, but AIP and AOPP had little effect on the ABA-induced mitigation of chilling stress. Na₂WO₄ and Flu diminished grafting- or SA-induced H₂O₂ biosynthesis, but DMTU and DPI did not affect ABA production induced by SA under chilling stress. These results suggest that SA participated in grafting-induced chilling tolerance by stimulating the biosynthesis of ABA and H₂O₂. H₂O₂, as a downstream signaler of ABA, mediates SA-induced chilling tolerance in grafted cucumber Plants.

abscisic acid; cucumber; hydrogen peroxide; pumpkin; salicylic acid; signal transduction chilling tolerance.