14-3-3 beta 蛋白, Human (His)

14-3-3 beta protein, functioning as an adapter, regulates diverse signaling pathways by recognizing phosphoserine or phosphothreonine motifs and modulating partner activity. It acts as a negative regulator in osteogenesis, inhibiting SRPK2's nuclear translocation and preventing neuronal apoptosis. Additionally, it negatively regulates MAP kinase-activating cascades through AKAP13. As a homodimer, it interacts with various proteins, emphasizing its diverse roles in cellular processes and signaling pathways. 14-3-3 beta Protein, Human (His) is the recombinant human-derived 14-3-3 beta protein, expressed by E. coli, with N-6*His labeled tag. The total length of 14-3-3 beta Protein, Human (His) is 246 a.a., with molecular weight of ~29 kDa.

14-3-3 β 蛋白作为衔接子参与广泛的一般和专门信号传导途径的调节。它识别磷酸丝氨酸或磷酸苏氨酸基序，与众多伴侣结合，在相互作用时调节结合伴侣的活性。它通过阻断 SRPK2 磷酸化形式的核转位，抵消其对细胞周期蛋白 D1 表达的刺激作用，从而防止 SRPK2 诱导的神经元凋亡，从而充当成骨负调节剂。此外，14-3-3 beta 负向调节通过 AKAP13 激活 MAP 激酶的信号级联。它以同二聚体形式存在，与多种蛋白质相互作用，包括 SAMSN1、PRKCE、AKAP13、SSH1、TORC2/CRTC2、ABL1、ROR2、GAB2、YAP1、SRPK2、PKA 磷酸化 AANAT、MYO1C、SIRT2、DAPK2、PI4KB、TBC1D22A、TBC1D22B 、SOS1、YWHAB、SLITRK1、SYNPO2、RIPOR2、MARK2、MARK3、TESK1、MEFV、HDAC4 和 ADAM22。这些相互作用凸显了 14-3-3 β 在各种细胞过程和信号通路中的不同作用。

Human

E. coli

N-6*His

P31946 (M1-N246)

7529  [NCBI]

MTMDKSELVQKAKLAEQAERYDDMAAAMKAVTEQGHELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTERNEKKQQMGKEYREKIEAELQDICNDVLELLDKYLIPNATQPESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLNEESYKDSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN

Approximately 29 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.