1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. B3GNT6 蛋白, Human (sf9, His)

B3GNT6 蛋白, Human (sf9, His)

目录号: HY-P76741
产品使用指南

B3GNT6蛋白是一种重要的β-1,3-N-乙酰氨基葡萄糖转移酶,对于O-聚糖核心3结构的合成至关重要,而O-聚糖是粘蛋白型糖蛋白生物合成所必需的。必需的。B3GNT6 在消化器官中发挥着关键作用,对糖复合物的结构多样性和功能特性做出了重大贡献。B3GNT6 蛋白, Human (sf9, His) 是重组的 B3GNT6 蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。

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描述

B3GNT6 protein is an important β-1,3-N-acetylglucosaminyltransferase, which is essential for the synthesis of O-glycan core 3 structure, and O-glycan is required for mucin-type glycoprotein biosynthesis. Required. B3GNT6 plays a critical role in digestive organs and contributes significantly to the structural diversity and functional properties of glycoconjugates. B3GNT6 Protein, Human (sf9, His) is the recombinant human-derived B3GNT6 protein, expressed by Sf9 insect cells , with N-His labeled tag.

研究背景

B3GNT6 蛋白充当重要的 β-1,3-N-乙酰氨基葡萄糖转移酶,专门负责 O-聚糖核心 3 结构的合成,该结构是粘蛋白型糖蛋白生物合成的重要前体。B3GNT6 在粘蛋白型 O-聚糖(尤其是消化器官中)的合成中发挥着关键作用,对糖复合物的结构多样性和功能特性做出了重大贡献。该酶参与核心 3 O-聚糖合成对于粘蛋白的正常形成和功能至关重要,影响与细胞粘附和粘膜保护相关的过程。了解 B3GNT6 的具体作用可以增强我们对糖基化途径的理解,为阐明消化器官生理学提供潜在的应用,并有助于探索聚糖相关的治疗靶点。对 B3GNT6 功能的进一步研究有望增进我们对粘蛋白型糖蛋白生物合成及其对健康和疾病的影响的了解。

种属

Human

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

Q6ZMB0-1 (Q44-S384)

基因 ID
蛋白结构
N-term
His
B3GNT6 (Q44-S384)
Accession # Q6ZMB0-1
C-term
同用名
Acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase; Core 3 synthase; Beta3Gn-T6
分子量

Approximately 47 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

B3GNT6 Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
B3GNT6 Protein, Human (sf9, His)
目录号:
HY-P76741
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