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  3. BAG-3 蛋白, Mouse (P.pastoris, His)

BAG-3 蛋白, Mouse (P.pastoris, His)

目录号: HY-P71848
产品使用指南

BAG-3蛋白是HSP70和HSC70的共伴侣,作为核苷酸交换因子促进ADP从这些伴侣中释放,从而触发底物释放。BAG-3 蛋白, Mouse (P.pastoris, His) 是重组的 BAG-3 蛋白,由 P. pastoris 表达,带有 N-His 标签。BAG-3 蛋白, Mouse (P.pastoris, His) 全长 576 个氨基酸,分子量约为 ~63.7 kDa。

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描述

The BAG-3 protein is a co-chaperone of HSP70 and HSC70, acting as a nucleotide exchange factor to promote the release of ADP from these partners, thus triggering substrate release. BAG-3 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived BAG-3 protein, expressed by P. pastoris , with N-His labeled tag. The total length of BAG-3 Protein, Mouse (P.pastoris, His) is 576 a.a., with molecular weight of ~63.7 kDa.

研究背景

BAG-3 蛋白与 HSP70 和 HSC70 伴侣蛋白一起充当辅助伴侣,作为核苷酸交换因子 (NEF) 发挥着关键作用。其 NEF 活性促进 HSP70 和 HSC70 释放 ADP,从而触发客户/底物蛋白的释放。核苷酸释放是通过其与 HSPA8/HSC70 的核苷酸结合域 (NBD) 的结合介导的,而底物释放是通过其与 HSPA8/HSC70 的底物结合域 (SBD) 的相互作用来促进的。除了在伴侣活性中的作用外,BAG-3 还表现出抗凋亡活性,并参与 HSF1 的核细胞质转运。它与 HSP70/HSC70 伴侣的 ATPase 结构域结合,与 BCL2、磷脂酶 C-gamma 蛋白和 DNAJB6 相互作用。此外,BAG-3 与 HSF1、HSPA8、HSPA1A 和 HSPA1B 相互作用,并通过其 WW 结构域 1 和 SYNPO2 的 PPPY 基序与 SYNPO2 结合,强调了其在细胞过程和蛋白质-蛋白质相互作用中的多功能参与。

种属

Mouse

表达系统

P. pastoris

标签

N-His

蛋白编号

Q9JLV1 (2S-577P)

基因 ID
蛋白结构
N-term
His
BAG-3 (2S-577P)
Accession # Q9JLV1
C-term
同用名
Bag3; Bis; MNCb-2243BAG family molecular chaperone regulator 3; BAG-3; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis
氨基酸序列

SAATQSPMMQMASGNGASDRDPLPPGWEIKIDPQTGWPFFVDHNSRTTTWNDPRVPPEGPKDTASSANGPSRDGSRLLPIREGHPIYPQLRPGYIPIPVLHEGSENRQPHLFHAYSQPGVQRFRTEAAAATPQRSQSPLRGGMTEAAQTDKQCGQMPATATTAAAQPPTAHGPERSQSPAASDCSSSSSSASLPSSGRSSLGSHQLPRGYIPIPVIHEQNITRPAAQPSFHQAQKTHYPAQQGEYQPQQPVYHKIQGDDWEPRPLRAASPFRSPVRGASSREGSPARSGTPVHCPSPIRVHTVVDRPQPMTHREPPPVTQPENKPESKPGPAGPDLPPGHIPIQVIRREADSKPVSQKSPPPAEKVEVKVSSAPIPCPSPSPAPSAVPSPPKNVAAEQKAAPSPAPAEPAAPKSGEAETPPKHPGVLKVEAILEKVQGLEQAVDSFEGKKTDKKYLMIEEYLTKELLALDSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEAEQPLQEIMGAVVADKDKKGPENKDPQTESQQLEAKAATPPNPSNPADSAGNLVAP

分子量

Approximately 63.7 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

BAG-3 Protein, Mouse (P.pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BAG-3 Protein, Mouse (P.pastoris, His)
目录号:
HY-P71848
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