CPLX2/Complexin-2 蛋白, Human (His)

Complexin-2 (CPLX2) protein plays a key role in regulating synaptic vesicle dynamics, acting as a negative regulator of synaptic vesicle cluster formation. It precisely affects vesicle localization to the presynaptic membrane. CPLX2/Complexin-2 Protein, Human (His) is the recombinant human-derived CPLX2/Complexin-2 protein, expressed by E. coli , with N-His labeled tag. The total length of CPLX2/Complexin-2 Protein, Human (His) is 133 a.a., with molecular weight of ~21 KDa.

Complexin-2 (CPLX2) 蛋白在调节有丝分裂后神经元内突触小泡动力学方面发挥着关键作用。它充当负调节剂，特别阻止活性区突触小泡簇的形成，从而影响小泡在突触前膜的精确定位。有趣的是，CPLX2还对胞吐作用的后期表现出积极的调节影响，参与各种细胞质囊泡的释放，包括突触囊泡和其他分泌囊泡。它的参与延伸到肥大细胞胞吐作用，强调了它在细胞过程中的多功能作用。值得注意的是，CPLX2 通过直接结合 SNARE 核心复合物（一个包含 SNAP25、VAMP2 和 STX1A 的分子整体）来实现这些调节作用，从而有助于突触处神经递质释放和囊泡动力学的复杂协调。

Human

E. coli

N-His

Q6PUV4 (D2-K134)

10814  [NCBI]

DFVMKQALGGATKDMGKMLGGEEEKDPDAQKKEEERQEALRQQEEERKAKHARMEAEREKVRQQIRDKYGLKKKEEKEAEEKAALEQPCEGSLTRPKKAIPAGCGDEEEEEEESILDTVLKYLPGPLQDMFKK

Approximately 21 kDa.

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.