1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  3. Ephrin/Eph Family Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Eph Receptors
  5. EphB4
  6. EphB4 蛋白, Human (sf9, His-GST)

EphB4 蛋白, Human (sf9, His-GST)

目录号: HY-P73003
产品使用指南

EphB4 蛋白是一种受体酪氨酸激酶,可与肝配蛋白 B 配体结合并启动双向信号传导。正信号传导调节细胞与表达 EFNB2 的细胞的排斥和分离。EphB4 蛋白, Human (sf9, His-GST) 是重组的 EphB4 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。

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描述

EphB4 protein is a receptor tyrosine kinase that binds to ephrin B ligand and initiates bidirectional signaling. Positive signaling regulates the rejection and separation of cells from EFNB2-expressing cells. EphB4 Protein, Human (sf9, His-GST) is the recombinant human-derived EphB4 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

研究背景

EphB4 蛋白是一种受体酪氨酸激酶,与位于相邻细胞上的跨膜肝配蛋白 B 家族配体混杂结合,启动接触依赖性双向信号传导。源自受体的下游途径称为正向信号传导,而肝配蛋白配体下游的信号传导途径称为反向信号传导。EphB4 与其同源配体 EFNB2 合作,复杂地参与调节细胞粘附和迁移。此外,EphB4 在心脏形态发生、血管生成以及血管重塑和通透性中发挥着核心作用。EPHB4 介导的正向信号传导有助于控制细胞排斥和与表达 EFNB2 的细胞的分离。

种属

Human

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

P54760-1 (L563-Y987)

基因 ID
蛋白结构
N-term
His-GST
EphB4 (L563-Y987)
Accession # P54760-1
C-term
同用名
Ephrin type-B receptor 4; EPHB4; HTK; MYK1; TYRO11
氨基酸序列

LRKQSNGREAEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY

分子量

Approximately 66 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
EphB4 Protein, Human (sf9, His-GST)
目录号:
HY-P73003
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