MCR-1 蛋白, E.coli (364a.a, His-SUMO)

The MCR-1 protein may catalyze the addition of a phosphoethanolamine moiety to lipid A, which is associated with polymyxin resistance. This modification observed in E. coli expressing MCR-1 increased the minimum inhibitory concentration (MIC) of colistin and polymyxin B. MCR-1 protein, E.coli (364a.a, His-SUMO) is the recombinant E. coli-derived MCR-1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of MCR-1 protein, E.coli (364a.a, His-SUMO) is 364 a.a., with molecular weight of ~56.7 kDa.

MCR-1 蛋白可能负责催化向脂质 A 添加磷酸乙醇胺部分，这是一种与多粘菌素抗性相关的修饰。这种修饰在赋予多粘菌素类抗生素耐药性方面发挥着至关重要的作用，表达 MCR-1 的大肠杆菌中粘菌素和多粘菌素 B 的最低抑制浓度 (MIC) 增加就证明了这一点。携带 mcr-1 基因的 pHNSHP45 质粒表现出向其他大肠杆菌菌株的高效转移性，并显着提高多粘菌素 MIC。值得注意的是，这种抵抗可能不需要细胞内维持的选择压力。当引入其他细菌物种（如肺炎克雷伯菌或铜绿假单胞菌）时，该质粒同样会增强多粘菌素 MIC。在一项使用来自人类患者的 mcr-1 编码质粒的小鼠大腿感染研究中，该质粒提供了针对粘菌素的体内保护。这些发现强调了 MCR-1 在介导各种细菌菌株对多粘菌素抗生素耐药性中的关键作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

N-His;N-SUMO

A0A0R6L508 (H178-R541)

55632978

HYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYHAKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSCGTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLPKAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAYFKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDVSMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDAITPTLLKLFDVTADKVKDRTAFIR

Approximately 56.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.