MERS-CoV Nucleoprotein/NP 蛋白 (sf9, His)

MERS-CoV Nucleoprotein/NP Protein is a phosphorylated basic protein and the second largest structural protein of MERS-CoV, containing 413 amino acid residues. The Nucleoprotein combines with the RNA genome to form a nucleocapsid, which is important in viral replication and assembly. In addition, Nucleoprotein plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. MERS-CoV Nucleoprotein/NP Protein (sf9, His) is the recombinant Virus-derived MERS-CoV Nucleoprotein/NP protein, expressed by Sf9 insect cells , with C-His labeled tag.

MERS-CoV 核蛋白/NP 是一种磷酸化碱性蛋白，是 MERS-CoV 的第二大结构蛋白，含有413个氨基酸残基。核蛋白与RNA基因组结合形成核衣壳，这对于病毒复制和组装非常重要。核蛋白与三联基序蛋白 25 (TRIM25) E3 泛素连接酶的 SPRY 结构域结合，从而干扰 TRIM25 与视黄酸诱导基因 I (RIG-I) 之间的关联，并抑制 TRIM25 介导的 RIG-I 泛素化和激活。因此，I 型干扰素的产生受到核蛋白的抑制。它将正链病毒基因组RNA包装成螺旋核糖核衣壳 (RNP)，并通过与病毒基因组和膜蛋白 M 的相互作用在病毒体组装过程中发挥重要作用。此外，核蛋白在提高亚基因组病毒效率方面也发挥着重要作用 RNA 转录以及病毒复制^[1][2]。

Virus

Sf9 insect cells

C-His

AFS88943.1 (M1-D413)

14254601  [NCBI]

Approximately 46.51 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% gly, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.