Nicastrin 蛋白, Mouse (HEK293, His)

Nicastrin is an important subunit of the γ-secretase complex and plays a key role in the endoproteolytic cleavage of integral membrane proteins, including Notch receptors and amyloid-β precursor protein (APP). In the γ-secretase complex, nicastin acts as a key regulator of Notch and Wnt signaling cascades and affects downstream processes. Nicastrin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Nicastrin protein, expressed by HEK293 , with C-His labeled tag. The total length of Nicastrin Protein, Mouse (HEK293, His) is 641 a.a., with molecular weight of 95-99 kDa.

尼卡斯特林是γ-分泌酶复合物中不可或缺的亚基，在负责膜蛋白内切蛋白水解的复杂分子机制中占据中心地位。这包括关键底物，如 Notch 受体和淀粉样β 前体蛋白 (APP)。尼卡斯特林 (Nicastrin) 在γ-分泌酶复合物中发挥着至关重要的作用，它是调节 Notch 和 Wnt 信号级联的关键，对下游过程发挥调节影响。尼卡斯特林与其他重要成分（例如早老素同型二聚体、APH1 和 PEN2）共同形成功能性 γ 分泌酶复合物。Nicastrin 的结合亲和力延伸至 APP 的蛋白水解加工的 C 末端片段，进一步强调了其在协调这种蛋白水解机制的复杂性中的关键作用。此外，尼卡斯特林与早老素成分的相互作用增强了其在γ-分泌酶复合物中的不可或缺的作用。

Mouse

HEK293

C-10*His

P57716 (G28-E668)

59287  [NCBI]

GLCGGNSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLKWVLTDGPNPPYMVLLEGKLFTRDVMEKLKGTTSRIAGLAVTLAKPNSTSSFSPSVQCPNDGFGIYSNSYGPEFAHCKKTLWNELGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFSHMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSVGLEPDVRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLSRNVMFVFFQGETFDYIGSSRMVYDMENGKFPVRLENIDSFVELGQVALRTSLDLWMHTDPMSQKNESVKNQVEDLLATLEKSGAGVPEVVLRRLAQSQALPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTAENINVTYPEWQSPEEDLNFVTDTAKALANVATVLARALYELAGGTNFSSSIQADPQTVTRLLYGFLVRANNSWFQSILKHDLRSYLDDRPLQHYIAVSSPTNTTYVVQYALANLTGKATNLTREQCQDPSKVPNESKDLYEYSWVQGPWNSNRTERLPQCVRSTVRLARALSPAFELSQWSSTEYSTWAESRWKDIQARIFLIASKELE

Approximately 95-120 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.