Nodal 蛋白, Mouse

Nodal protein plays a key role in embryonic development and is integral for mesoderm formation and axial patterning. Its involvement in these fundamental processes underscores its importance in coordinating the complex series of events that determine early embryonic morphogenesis. Nodal Protein, Mouse is the recombinant mouse-derived Nodal protein, expressed by E. coli , with tag free.

节点蛋白是胚胎发育的关键参与者，是中胚层形成和轴向模式的关键因素。该蛋白质作为同二聚体发挥作用，形成坚固的分子结构，其中两个相同的亚基通过二硫键错综复杂地连接。由这种蛋白质介导的节点信号通路有助于指导基本过程，例如细胞分化为中胚层谱系以及发育胚胎中轴向模式的建立。这种调节作用强调了节点蛋白在胚胎发生过程中协调细胞事件的复杂舞蹈中的重要性，强调了它对生命早期阶段组织和结构的正确组织和发育的重要贡献。（参考文献：胚胎发育过程中中胚层形成和轴向模式所必需的。同源二聚体；二硫键连接。）

Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Approximately 100 ng/mL of Recombinant Mouse Nodal can effictively induce Smad2 phosphorylation.

Mouse

E. coli

Tag Free

P43021 (H245-L354)

18119  [NCBI]

HHLPDRSQLCRRVKFQVDFNLIGWGSWIIYPKQYNAYRCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLEHHKDMIVEECGCL

Approximately 16 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 4 mM HCL, pH 2.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 50 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.