PTS 蛋白, Human (His)

PTS proteins are critical in the biosynthesis of tetrahydrobiopterin, an important cofactor for aromatic amino acid hydroxylase. It catalyzes the conversion of 7,8-dihydroneopterin triphosphate to 6-pyruvoyltetrahydropterin, a key step in the biosynthetic pathway. PTS Protein, Human (His) is the recombinant human-derived PTS protein, expressed by E. coli , with N-His labeled tag. The total length of PTS Protein, Human (His) is 145 a.a., with molecular weight of ~17 kDa.

PTS 蛋白在四氢生物蝶呤的生物合成中起着至关重要的作用，四氢生物蝶呤是芳香族氨基酸羟化酶的重要辅助因子。该酶有助于催化 7,8-二氢新蝶呤三磷酸转化为 6-丙酮酰四氢蝶呤，这是生物合成途径的关键步骤。PTS 蛋白的催化活性强调了其在促进四氢生物蝶呤生产所需的转化方面的重要性，而四氢生物蝶呤对于芳香族氨基酸羟化酶的正常运作至关重要。

Human

E. coli

N-His

Q03393 (M1-E145)

5805  [NCBI]

MSTEGGGRRCQAQVSRRISFSASHRLYSKFLSDEENLKLFGKCNNPNGHGHNYKVVVTVHGEIDPATGMVMNLADLKKYMEEAIMQPLDHKNLDMDVPYFADVVSTTENVAVYIWDNLQKVLPVGVLYKVKVYETDNNIVVYKGE

Approximately 17 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, 40% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.