TDT 蛋白, Bos taurus (His)

The TDT protein is a template-independent DNA polymerase that randomly adds deoxynucleoside 5'-triphosphates to the 3' end of the DNA initiator. Notably, its in vivo effects involve diversifying the immunoglobulin and T-cell receptor repertoire by adding nucleotides at the junction (N-region) of rearranged gene segments during B- and T-cell maturation. TDT Protein, Bos taurus (His) is the recombinant TDT protein, expressed by E. coli , with N-6*His labeled tag. TDT Protein, Bos taurus (His), has molecular weight of ~58.3 kDa.

TDT 蛋白作为不依赖于模板的 DNA 聚合酶，催化脱氧核苷 5'-三磷酸随机添加到 DNA 起始子的 3' 端，这一过程已在科学文献中记录。值得注意的是，其体内作用之一涉及在 B 细胞和 T 细胞的成熟阶段，在重排的免疫球蛋白 (Ig) 重链和 T 细胞受体基因片段的连接处（N 区）添加核苷酸。这强调了 TDT 对免疫球蛋白和 T 细胞受体库多样化的特殊贡献，阐明了其在 B 细胞和 T 细胞成熟过程中形成适应性免疫反应的重要作用。

Others

E. coli

N-6*His

P06526 (M1-A509)

281120  [NCBI]

MDPLCTASSGPRKKRPRQVGASMASPPHDIKFQNLVLFILEKKMGTTRRNFLMELARRKGFRVENELSDSVTHIVAENNSGSEVLEWLQVQNIRASSQLELLDVSWLIESMGAGKPVEITGKHQLVVRTDYSATPNPGFQKTPPLAVKKISQYACQRKTTLNNYNHIFTDAFEILAENSEFKENEVSYVTFMRAASVLKSLPFTIISMKDTEGIPCLGDKVKCIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRSLSKIMSDKTLKFTKMQKAGFLYYEDLVSCVTRAEAEAVGVLVKEAVWAFLPDAFVTMTGGFRRGKKIGHDVDFLITSPGSAEDEEQLLPKVINLWEKKGLLLYYDLVESTFEKFKLPSRQVDTLDHFQKCFLILKLHHQRVDSSKSNQQEGKTWKAIRVDLVMCPYENRAFALLGWTGSRQFERDIRRYATHERKMMLDNHALYDKTKRVFLKAESEEEIFAHLGLDYIEPWERNA

Approximately 58.3 kDa

Greater than 80% as determined by reducing SDS-PAGE.