Thioredoxin/TXN 蛋白, Human

Thioredoxin/TXN Protein participates in diverse redox reactions, using its active center dithiol for reversible oxidation and disulfide bond formation. It catalyzes crucial dithiol-disulfide exchanges and plays a pivotal role in S-nitrosylation of cysteine residues, responding to intracellular nitric oxide. Thioredoxin regulates caspase-3 activity by nitrosylating CASP3's active site cysteine. It also influences FOS/JUN AP-1 DNA binding and augments interleukin-2 receptor expression, showcasing its multifaceted cellular role beyond redox regulation. Thioredoxin/TXN Protein, Human is the recombinant human-derived Thioredoxin/TXN protein, expressed by E. coli, with tag free. The total length of Thioredoxin/TXN Protein, Human is 105 a.a., with molecular weight of ~14 kDa.

硫氧还蛋白/TXN 蛋白积极参与多种氧化还原反应，利用其活性中心二硫醇的可逆氧化形成二硫键，并催化关键的二硫醇-二硫键交换反应。除了其经典的氧化还原功能外，硫氧还蛋白在靶蛋白内半胱氨酸残基的可逆 S-亚硝基化中发挥着关键作用，有助于细胞对细胞内一氧化氮的反应。值得注意的是，它通过响应一氧化氮对 CASP3 活性位点半胱氨酸进行亚硝基化来对 caspase-3 活性进行调节控制。此外，硫氧还蛋白显示其对电离辐射细胞中 FOS/JUN AP-1 DNA 结合活性的影响，通过其氧化/还原状态调节 AP-1 转录活性。此外，硫氧还蛋白还与白细胞介素 2 受体 TAC (IL2R/P55) 表达的增强有关，突出了其在氧化还原调节之外的细胞过程中的多方面作用。

Measured by its ability to catalyze the reduction of insulin. The reaction leads toprecipitation, which can be measured by absorbance at 650 nm and the specific activity is 5-10 A650/min/mg.

Human

E. coli

Tag Free

P10599 (M1-V105)

7295

MVKQIESKTAFQEALDAAGDKLVVVDFSATWCGPCKMIKPFFHSLSEKYSNVIFLEVDVDDCQDVASECEVKCMPTFQFFKKGQKVGEFSGANKEKLEATINELV

Approximately 14 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.