1. 重组蛋白
  2. CD Antigens
  3. Macrophage CD Proteins Stem Cell CD Proteins Endothelial cell CD Proteins
  4. Thy-1/CD90
  5. Thy1/CD90 蛋白, Rat (HEK293, His)

Thy1/CD90 蛋白, Rat (HEK293, His)

目录号: HY-P74514
COA 产品使用指南

Thy1/CD90 蛋白可能在与神经发育相关的关键细胞过程中发挥作用,特别是在突触发生和其他大脑事件期间,表明它参与细胞-细胞或细胞-配体相互作用。Thy1/CD90 蛋白, Rat (HEK293, His) 是重组的 Thy1/CD90 蛋白,由 HEK293 表达,带有 C-His 标签。Thy1/CD90 蛋白, Rat (HEK293, His) 全长 110 个氨基酸,分子量约为 ~23-27 kDa。

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100 μg ¥6000 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The Thy1/CD90 protein may play a role in key cellular processes associated with neurodevelopment, particularly during synaptogenesis and other brain events, suggesting its involvement in cell-cell or cell-ligand interactions. Thy1/CD90 Protein, Rat (HEK293, His) is the recombinant rat-derived Thy1/CD90 protein, expressed by HEK293 , with C-His labeled tag. The total length of Thy1/CD90 Protein, Rat (HEK293, His) is 110 a.a., with molecular weight of ~23-27 kDa.

研究背景

Thy1/CD90 蛋白被认为可能在细胞-细胞或细胞-配体相互作用中发挥作用,特别是在突触发生和大脑中的其他事件期间,表明它参与与神经发育和突触连接相关的关键过程。Thy1/CD90 在这些事件期间运作的精确机制和特定环境仍然是人们感兴趣的领域,强调了其在大脑复杂景观中协调细胞相互作用的潜在重要性。Thy1/CD90 在介导细胞通讯和潜在影响突触形成方面的多功能性强调需要进一步探索以阐明其在这些动态过程中的确切功能和分子机制。

生物活性

Measured by its binding ability in a functional ELISA. When Recombinant Rat CD90 is immobilized at 5.00 µg/mL (100 µL/well) can bind Human Galectin-1. The ED50 for this effect is 0.3630 μg/mL.

种属

Rat

表达系统

HEK293

标签

C-His

蛋白编号

P01830 (Q20-K129)

基因 ID
蛋白结构
N-term
Thy1 (Q20-K129)
Accession # P01830
His
C-term
同用名
Thy-1 membrane glycoprotein; CDw90; Thy-1 antigen; CD90
氨基酸序列

QRVISLTACLVNQNLRLDCRHENNTNLPIQHEFSLTREKKKHVLSGTLGVPEHTYRSRVNLFSDRFIKVLTLANFTTKDEGDYMCELRVSGQNPTSSNKTINVIRDKLVK

分子量

Approximately 23-27 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Thy1/CD90 Protein, Rat (HEK293, His)
目录号:
HY-P74514
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