Arbutin (Synonyms: 熊果苷; β-Arbutin)

目录号: HY-N0192 纯度: 99.76%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Arbutin (β-Arbutin) 是一种广泛存在的天然多酚，具有抗氧化，抗炎和抗肿瘤特性。是黑色素细胞中酪氨酸酶 (tyrosinase) 的竞争性抑制剂，对单酚酶的 K_i^app 值为 1.42 mM；对二酚酶 K_i^app 的值为 0.9 mM。Arbutin 也用作脱色剂。

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Arbutin Chemical Structure

CAS No. : 497-76-7

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥500	In-stock
500 mg	￥400	In-stock
5 g	￥550	In-stock
10 g	￥910	In-stock
50 g		询价

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Customer Review

Other Forms of Arbutin:

Arbutin-d₄ 询价
Arbutin (Standard) 询价

MCE 顾客使用本产品发表的 1 篇科研文献

•FEBS Open Bio. 2021 Jan;11(1):289-299. [Abstract]

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生物活性
纯度 & 产品资料
参考文献

生物活性

Arbutin (β-Arbutin) is a competitive inhibitor of tyrosinase, with K_i^app values of 1.42 mM for monophenolase; 0.9 mM for diphenolase. Arbutin is also used as depigmenting agents^[1]. Arbutin is a natural polyphenol isolated from the bearberry plant Arctostaphylos uvaursi, possesses with anti-oxidant, anti-inflammatory and anti-tumor properties^[2]^[3].

IC₅₀ & Target

Human Endogenous Metabolite

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
B16	IC₅₀	116 μM Compound: Arbutin	Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs	[PMID: 19615910]
B16	IC₅₀	120 μM Compound: Arbutin	Inhibition of alpha-MSH -stimulated melanogenesis in mouse B16 cells assessed as melanin release after 3 days Inhibition of alpha-MSH -stimulated melanogenesis in mouse B16 cells assessed as melanin release after 3 days	[PMID: 21388810]
B16	IC₅₀	140 μM Compound: Arbutin	Inhibition of melanin production in alpha-MSH stimulated mouse B16 cells after 72 hrs by spectrophotometry Inhibition of melanin production in alpha-MSH stimulated mouse B16 cells after 72 hrs by spectrophotometry	[PMID: 23623417]
B16	IC₅₀	1550 μM Compound: 2	Inhibition of tyrosinase activity in alpha-MSH-stimulated mouse B16 cells using L-tyrosine as substrate Inhibition of tyrosinase activity in alpha-MSH-stimulated mouse B16 cells using L-tyrosine as substrate	[PMID: 21429753]
B16	IC₅₀	180 μM Compound: Arbutin	Antimelanogenic activity at alpha-MSH-stimulated mouse melanoma B16 cells after 3 days by spectrophotometry Antimelanogenic activity at alpha-MSH-stimulated mouse melanoma B16 cells after 3 days by spectrophotometry	[PMID: 22217872]
B16	IC₅₀	180 μM Compound: Arbutin	Inhibition of alpha-MSH-induced melanin production in mouse B16 cells after 3 days Inhibition of alpha-MSH-induced melanin production in mouse B16 cells after 3 days	[PMID: 21601449]
B16	IC₅₀	180 μM Compound: arbutin	Inhibition of alpha-MSH-induced melanin production in mouse B16 cells after 72 hrs Inhibition of alpha-MSH-induced melanin production in mouse B16 cells after 72 hrs	[PMID: 20097083]
B16	IC₅₀	180 μM Compound: Arbutin	Inhibition of alpha-MSH-induced melanin production in mouse B16 cells after 72 hrs Inhibition of alpha-MSH-induced melanin production in mouse B16 cells after 72 hrs	[PMID: 20044259]
B16	IC₅₀	247 μM Compound: Arbutin	Inhibition of melanin production in alpha-MSH-stimulated mouse B16 cells incubated for 24 hrs followed by alpha-MSH stimulation for 72 hrs in presence of compound Inhibition of melanin production in alpha-MSH-stimulated mouse B16 cells incubated for 24 hrs followed by alpha-MSH stimulation for 72 hrs in presence of compound	[PMID: 29526485]
B16	IC₅₀	64 μM Compound: Arbutin	Inhibition of alpha-MSH-stimulated melanogenesis in mouse B16 cells assessed as reduction in melanin production incubated for 3 days Inhibition of alpha-MSH-stimulated melanogenesis in mouse B16 cells assessed as reduction in melanin production incubated for 3 days	[PMID: 31387790]
B16	IC₅₀	67.9 μg/mL Compound: Arbutin	Inhibition of alpha-MSH-stimulated melanogenesis in mouse B16 cells assessed as melanin release after 72 hrs Inhibition of alpha-MSH-stimulated melanogenesis in mouse B16 cells assessed as melanin release after 72 hrs	[PMID: 22450129]
B16	IC₅₀	99 μM Compound: 2	Inhibition of alpha-MSH-induced melanin synthesis in mouse B16 cells grown in a 5% CO2-95% air atmosphere after 3 days Inhibition of alpha-MSH-induced melanin synthesis in mouse B16 cells grown in a 5% CO2-95% air atmosphere after 3 days	[PMID: 21429753]
B16-4A5	IC₅₀	> 1000 μM Compound: Arbutin	Inhibition of theophylline-stimulated mouse B16-4A5 cell proliferation assessed as cell viability after 68 hrs by WST8 assay Inhibition of theophylline-stimulated mouse B16-4A5 cell proliferation assessed as cell viability after 68 hrs by WST8 assay	[PMID: 19615910]
B16-4A5	IC₅₀	174 μM Compound: Arutin	Inhibition of melanogenesis in mouse B16-4A5 cells after 72 hrs by spectrophotometry Inhibition of melanogenesis in mouse B16-4A5 cells after 72 hrs by spectrophotometry	[PMID: 23270663]
B16-4A5	IC₅₀	174 μM Compound: arbutin	Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs by microplate reader analysis Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs by microplate reader analysis	[PMID: 25987378]
B16-4A5	IC₅₀	174 μM Compound: Arbutin	Inhibition of melanogenesis in mouse B16-4A5 cells after 72 hrs by spectrophotometry relative to control Inhibition of melanogenesis in mouse B16-4A5 cells after 72 hrs by spectrophotometry relative to control	[PMID: 24601675]
B16-4A5	IC₅₀	174 μM Compound: Arbutin	Inhibition of theophylline-induced melanogenesis in mouse B16-4A5 cells after 72 hrs by spectrophotometric analysis relative to control Inhibition of theophylline-induced melanogenesis in mouse B16-4A5 cells after 72 hrs by spectrophotometric analysis relative to control	[PMID: 23376010]
B16-4A5	IC₅₀	174 μM Compound: Arbutin	Inhibition of theophylline-stimulated melanogenesis in mouse B16-4A5 cells after 72 hrs Inhibition of theophylline-stimulated melanogenesis in mouse B16-4A5 cells after 72 hrs	[PMID: 20189399]
B16-F1	IC₅₀	122 μM Compound: Arbutin	Inhibition of melanin production in C57BL/6J mouse B16-F1 cells incubated for 5 days Inhibition of melanin production in C57BL/6J mouse B16-F1 cells incubated for 5 days	[PMID: 27720556]
B16-F1	IC₅₀	148.3 μM Compound: Arbutin	Antimelanogenic activity in mouse B16-F1 cells assessed as reduction in alpha-MSH-stimulated melanin production preincubated for 72 hrs followed by alpha-MSH-stimulation and measured after 2 hrs Antimelanogenic activity in mouse B16-F1 cells assessed as reduction in alpha-MSH-stimulated melanin production preincubated for 72 hrs followed by alpha-MSH-stimulation and measured after 2 hrs	[PMID: 30446440]
B16-F10	IC₅₀	391.2 μM Compound: Arbutin	Cytotoxicity against mouse B16-F10 cells assessed as reduction in cell viability incubated for 72 hrs by XTT assay Cytotoxicity against mouse B16-F10 cells assessed as reduction in cell viability incubated for 72 hrs by XTT assay	[PMID: 27720556]
Melan-a	IC₅₀	98.05 μM Compound: Arbutin	Inhibition of melanin formation in mouse Melan-a cells after 5 days by ELISA Inhibition of melanin formation in mouse Melan-a cells after 5 days by ELISA	[PMID: 20022495]

体外研究
(In Vitro)

Arbutin (0.3-5.4 mM；24 小时、48 小时、72 小时；B16 小鼠黑色素瘤细胞) 以时间和剂量依赖性方式抑制 B16 小鼠黑色素瘤细胞的活力^[2]。
Arbutin (1.4-5.4 mM；24 小时) 以 5.4 mM的剂量增加处理的 B16 鼠黑色素瘤细胞的凋亡率^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	B16 murine melanoma cells
Concentration:	0.3 mM, 0.7 mM, 1.4 mM, 2.1 mM, 2.9 mM, 3.6 mM, 5.4 mM
Incubation Time:	24 hours, 48 hours or 72 hours
Result:	Inhibited the viability of B16 murine melanoma cells in a time- and dose-dependent manner.

Apoptosis Analysis^[2]

Cell Line:	B16 murine melanoma cells
Concentration:	1.4 mM, 2.9 mM, 5.4 mM
Incubation Time:	24 hours
Result:	Caused apoptosis in 19.3% of the cells.

体内研究
(In Vivo)

Arbutin (50 mg/kg、100 mg/kg；口服；每天；17 天；雄性 C57BL/6 小鼠) 预处理对 ISO 诱导的小鼠心脏肥大具有显著的保护作用^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6 mice ( 20-25 g)^[3]
Dosage:	50 mg/kg, 100 mg/kg
Administration:	Oral administration; every day; for 17 days
Result:	Ameliorated the ISO-induced myocardial injury.

分子量

272.25

Formula

C₁₂H₁₆O₇

CAS 号

497-76-7

性状

固体

颜色

White to off-white

中文名称

熊果苷；熊果酚甙；熊果素；熊果叶甙；楊梅葉甘

结构分类

Phenols

初始来源

微生物

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : ≥ 50 mg/mL (183.65 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H₂O 中的溶解度 : 33.33 mg/mL (122.42 mM; 超声助溶)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.6731 mL	18.3655 mL	36.7309 mL
5 mM	0.7346 mL	3.6731 mL	7.3462 mL
10 mM	0.3673 mL	1.8365 mL	3.6731 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.18 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.18 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.18 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (367.31 mM); 澄清溶液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

该产品水溶性佳，请具体参考实测水 / PBS / Saline 中的溶解度数据。

您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。

纯度 & 产品资料

纯度: 99.76%

选择批次：

Data Sheet (638 KB) SDS (394 KB)

COA (192 KB) HNMR (140 KB) RP-HPLC (205 KB) MS (71 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Garcia-Jimenez A, et al. Action of tyrosinase on alpha and beta-arbutin: A kinetic study. PLoS One. 2017 May 11;12(5):e0177330. [Content Brief]

[2]. Jiang L, et al. Investigation of the pro-apoptotic effects of arbutin and its acetylated derivative on murinemelanoma cells. Int J Mol Med. 2018 Feb;41(2):1048-1054. [Content Brief]

[3]. Nalban N, et al. Arbutin Attenuates Isoproterenol-Induced Cardiac Hypertrophy by Inhibiting TLR-4/NF-κB Pathway in Mice. Cardiovasc Toxicol. 2019 Sep 4. [Content Brief]

Arbutin 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.6731 mL	18.3655 mL	36.7309 mL	91.8274 mL
	5 mM	0.7346 mL	3.6731 mL	7.3462 mL	18.3655 mL
	10 mM	0.3673 mL	1.8365 mL	3.6731 mL	9.1827 mL
	15 mM	0.2449 mL	1.2244 mL	2.4487 mL	6.1218 mL
	20 mM	0.1837 mL	0.9183 mL	1.8365 mL	4.5914 mL
	25 mM	0.1469 mL	0.7346 mL	1.4692 mL	3.6731 mL
	30 mM	0.1224 mL	0.6122 mL	1.2244 mL	3.0609 mL
	40 mM	0.0918 mL	0.4591 mL	0.9183 mL	2.2957 mL
	50 mM	0.0735 mL	0.3673 mL	0.7346 mL	1.8365 mL
	60 mM	0.0612 mL	0.3061 mL	0.6122 mL	1.5305 mL
	80 mM	0.0459 mL	0.2296 mL	0.4591 mL	1.1478 mL
	100 mM	0.0367 mL	0.1837 mL	0.3673 mL	0.9183 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Arbutin497-76-7β-Arbutin熊果苷熊果酚甙熊果素熊果叶甙楊梅葉甘TyrosinaseEndogenous MetaboliteInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

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上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Arbutin (Synonyms: 熊果苷; β-Arbutin)

Customer Review

Other Forms of Arbutin:

MCE 顾客使用本产品发表的 1 篇科研文献