2. Cell Cycle/DNA Damage Autophagy Anti-infection NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. Topoisomerase Autophagy Bacterial Reactive Oxygen Species Antibiotic Endogenous Metabolite Parasite
  4. Berberine chloride hydrate

Berberine chloride hydrate  (Synonyms: 盐酸黄连素水合物; Natural Yellow 18 chloride hydrate)

目录号: HY-17577 纯度: 99.78%
COA 产品使用指南

摩尔计算器

Berberine chloride hydrate (Natural Yellow 18 chloride hydrate) 是一种生物碱，常用作抗生素。Berberine chloride hydrate 诱导活性氧 (ROS) 生成并抑制 DNA 拓扑异构酶 (topoisomerase)。抗肿瘤特性。

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Berberine chloride hydrate Chemical Structure

Berberine chloride hydrate Chemical Structure

CAS No. : 68030-18-2

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥726
In-stock
100 mg ￥660
In-stock
1 g ￥1500
In-stock
5 g ￥3500
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Customer Review

Other Forms of Berberine chloride hydrate:

Berberine chloride hydrate 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 45 篇科研文献

WB
Proliferation Assay
Cell Viability Assay

    Berberine chloride hydrate purchased from MCE. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29.  [Abstract]

    Berberine (BBR; 0.625, 1.25, 2.5, 5, 10 µM; 48 h) inhibits the viability of A431cells in a dose-dependent matter.

    Berberine chloride hydrate purchased from MCE. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29.  [Abstract]

    Berberine (BBR; 2.5, 5, 10 µM; 48 h) inhibits EGFR phosphorylation in a dose-dependent matter in A431cells.

    Berberine chloride hydrate purchased from MCE. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29.  [Abstract]

    Berberine (BBR; 2.5 µM; 10 days) significantly inhibits NCI-H441 colony formation.

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    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Berberine chloride hydrate (Natural Yellow 18 chloride hydrate) is an alkaloid that acts as an antibiotic. Berberine chloride hydrate induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Antineoplastic properties[1].

    IC50 & Target

    ROS[1]
    DNA topoisomerase[1]
    体外研究
    (In Vitro)

    Berberine chloride hydrate ( Natural Yellow 18 chloride hydrate; 1.25-160 μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29[1].
    Berberine chloride hydrate (1.25-160 μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth[1].
    LoVo cells are exposure to Berberine chloride hydrate (10-80 μM) for 24 h. Cell cycle analysis of 40 μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase[1].
    Berberine chloride hydrate (10-80 μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0 μM[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Berberine chloride hydrate 相关抗体:

    Cell Proliferation Assay[1]

    Cell Line: Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29
    Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
    Incubation Time: 72 hours
    Result: Inhibited the proliferation of four cell lines. The IC50 ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116).

    Cell Proliferation Assay[1]

    Cell Line: Colorectal carcinoma cell lines LoVo
    Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
    Incubation Time: 24, 48, 72 hours
    Result: Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells.

    Cell Cycle Analysis[1]

    Cell Line: LoVo cells
    Concentration: 0, 10, 20, 40, or 80 μM
    Incubation Time: 24 hours
    Result: Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.

    Western Blot Analysis[1]

    Cell Line: LoVo cells
    Concentration: 10, 20, 40, or 80 μM
    Incubation Time: 24 hours
    Result: Suppressed cyclin B1, cdc2 and cdc25c protein expression.
    体内研究
    (In Vivo)

    Berberine chloride hydrate (Natural Yellow 18 chloride hydrate; 10, 30, or 50 mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine chloride hydrate at doses of 30 and 50 mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts[1]
    Dosage: 10, 30, or 50 mg/kg/day
    Administration: Gastrointestinal gavage; for 10 consecutive days
    Result: Showed inhibitory rates of 33.1 % and 45.3 % at doses of 30 and 50 mg/kg/day.
    Clinical Trial
    分子量

    389.83

    Formula

    C20H20ClNO5
    CAS 号
    性状

    固体

    颜色

    Light yellow to yellow

    中文名称

    盐酸小檗碱水合物；盐酸黄连素水合物
    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : ≥ 3.9 mg/mL (10.00 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    H2O 中的溶解度 : 1 mg/mL (2.57 mM; 超声助溶 (<60°C))

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.5652 mL 12.8261 mL 25.6522 mL
    5 mM 0.5130 mL 2.5652 mL 5.1304 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料

    纯度: 99.82%

    COA (270 KB) LCMS (118 KB)

    产品使用指南 (1538 KB)
    参考文献

    Berberine chloride hydrate 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.5652 mL 12.8261 mL 25.6522 mL 64.1305 mL
    DMSO 5 mM 0.5130 mL 2.5652 mL 5.1304 mL 12.8261 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Berberine chloride68030-18-2Natural Yellow 18 chloride盐酸小檗碱水合物盐酸黄连素水合物TopoisomeraseAutophagyBacterialReactive Oxygen SpeciesAntibioticEndogenous MetaboliteParasiteInhibitorinhibitorinhibit

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    目录号：
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