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BD-2 蛋白, Human

目录号: HY-P7135
COA 产品使用指南

BD-2 蛋白, Human是一种参与对抗微生物入侵反应的抗微生物肽。

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规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥440 In-stock
10 μg ¥700 In-stock
50 μg ¥1800 In-stock
100 μg ¥2880 询价
> 100 μg   询价  

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BD-2 Protein, Human 相关产品:

MCE 顾客使用本产品发表的科研文献

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

BD-2 Protein, Human is an anti-microbial peptide that participates in the response to microbial invasion.

研究背景

人类 β-防御素-2 (HBD-2) 是一种有效的抗微生物肽,是先天免疫反应的一部分。HBD-2 是一种存在于羊水中的天然抗菌肽,参与宿主对羊膜腔微生物入侵的反应[1]。与 hBD-1 不同,第二种肽,人 β-防御素-2 (hBD-2),在转录水平上受到与微生物接触的反应而受到调节,并且在杀死革兰氏阴性细菌方面非常有效。细胞因子肿瘤坏死因子-α[2] 也会上调其表达。

生物活性

1.Full biological activity determined by a chemotaxis bioassay using immature human dendritic cells is in a concentration range of 10-100 ng/mL.
2.Measured by its ability to chemoattract THP-1 cells. The ED50 for this effect is 65.46 ng/mL, corresponding to a specific activity is 1.528×104U/mg.
3.Measured by its ability to chemoattract immature human dendritic cells. The ED50 for this effect is 65.16 ng/mL, corresponding to a specific activity is 1.535×104 U/mg.

  • Measured by its ability to chemoattract THP-1 cells. The ED50 for this effect is 65.46 ng/mL, corresponding to a specific activity is 1.528×104 U/mg.
种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

O15263 (G24-P64)

基因 ID
蛋白结构
N-term
BD-2 (G24-P64)
Accession # O15263
C-term
中文名
重组人 β-防御素-2
同用名
rHuBD-2; DEFB2
氨基酸序列

GIGDPVTCLKSGAICHPVFCPRRYKQIGTCGLPGTKCCKKP

分子量

Approximately 4.3-9 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized after extensive dialysis against 20 mM PBS, pH 7.4, 130 mM NaCl or PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

BD-2 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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