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  3. Chitinase-like 蛋白 3/YM1 蛋白, Mouse (HEK293, His)

Chitinase-like 蛋白 3/YM1 蛋白, Mouse (HEK293, His)

目录号: HY-P7845
COA 产品使用指南

几丁质酶样蛋白 3/YM1 作为凝集素发挥作用,对含有游离氨基的糖类,特别是寡聚糖类具有很强的亲和力。它与几丁质和肝素结合,表现出弱己糖胺酶活性,而没有几丁质酶活性。值得注意的是,该蛋白质对 T 淋巴细胞、骨髓细胞和嗜酸性粒细胞表现出趋化活性,表明其在炎症和过敏中具有潜在作用。Chitinase-like 蛋白 3/YM1 蛋白, Mouse (HEK293, His) 是重组的 几丁质酶样蛋白 3/YM1 蛋白,由 HEK293 表达,带有 C-6*His 标签。Chitinase-like 蛋白 3/YM1 蛋白, Mouse (HEK293, His) 全长 377 个氨基酸,分子量为 41-45 kDa。

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规格 价格 是否有货 数量
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5 μg ¥470 In-stock
10 μg ¥800 In-stock
50 μg ¥2250 In-stock
100 μg ¥3800 询价
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Chitinase-like protein 3/YM1, functioning as a lectin, demonstrates a strong affinity for saccharides containing free amino groups, particularly oligomeric saccharides. It binds to chitin and heparin, displaying weak hexosaminidase activity without chitinase activity. Notably, the protein exhibits chemotactic activity for T-lymphocytes, bone marrow cells, and eosinophils, indicating potential roles in inflammation and allergies. Chitinase-like protein 3/YM1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Chitinase-like protein 3/YM1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Chitinase-like protein 3/YM1 Protein, Mouse (HEK293, His) is 377 a.a., with molecular weight of 41-45 kDa.

研究背景

几丁质酶样蛋白 3/YM1 蛋白起到凝集素的作用,对含有游离氨基的糖类(包括葡萄糖胺或半乳糖胺)具有显着的亲和力。与单糖或二糖相比,其对低聚糖的结合亲和力要强得多。此外,几丁质酶样蛋白 3/YM1 与几丁质和肝素结合。虽然它表现出弱己糖胺酶活性,但它不具有几丁质酶活性。除了其酶促功能外,该蛋白还对 T 淋巴细胞、骨髓细胞和嗜酸性粒细胞表现出趋化活性,表明其可能参与炎症和过敏过程。

生物活性

Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED50 for this effect is 0.9299 μg/mL, corresponding to a specific activity is 1075.3844 units/mg.

  • Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED50 for this effect is 0.9299 μg/mL, corresponding to a specific activity is 1075.3844 units/mg.
种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

O35744 (Y22-Y398)

基因 ID

12655  [NCBI]

蛋白结构
N-term
CHIL3 (Y22-Y398)
Accession # O35744
6*His
C-term
同用名
rMuChitinase-like protein 3/YM1, His; Chitinase-like protein 3; Chil3
氨基酸序列

YQLMCYYTSWAKDRPIEGSFKPGNIDPCLCTHLIYAFAGMQNNEITYTHEQDLRDYEALNGLKDKNTELKTLLAIGGWKFGPAPFSAMVSTPQNRQIFIQSVIRFLRQYNFDGLNLDWQYPGSRGSPPKDKHLFSVLVKEMRKAFEEESVEKDIPRLLLTSTGAGIIDVIKSGYKIPELSQSLDYIQVMTYDLHDPKDGYTGENSPLYKSPYDIGKSADLNVDSIISYWKDHGAASEKLIVGFPAYGHTFILSDPSKTGIGAPTISTGPPGKYTDESGLLAYYEVCTFLNEGATEVWDAPQEVPYAYQGNEWVGYDNVRSFKLKAQWLKDNNLGGAVVWPLDMDDFSGSFCHQRHFPLTSTLKGDLNIHSASCKGPY

分子量

41-45 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Chitinase-like protein 3/YM1 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Chitinase-like protein 3/YM1 Protein, Mouse (HEK293, His)
目录号:
HY-P7845
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