ERK2 蛋白, Human (sf9, His)

ERK2 Protein, a key component of the MAPK/ERK cascade, regulates diverse cellular processes such as transcription, translation, mitosis, apoptosis, endosomal dynamics, and Golgi apparatus fragmentation. Through phosphorylation, it modulates substrates including transcription factors, cytoskeletal elements, apoptosis regulators, translation regulators, protein kinases, and phosphatases. ERK2 Protein, Human (sf9, His) is the recombinant human-derived ERK2 protein, expressed by Sf9 insect cells , with C-His labeled tag.

MAPK/ERK 级联在多种细胞过程的调节中发挥着至关重要的作用，包括转录、翻译、有丝分裂、细胞凋亡、内体动力学和高尔基体碎片。它通过磷酸化多种底物来实现这一目标，包括转录因子、细胞骨架元件、细胞凋亡调节因子、翻译调节因子、蛋白激酶和磷酸酶。

Measured by its ability to catalyze 250 μg/mL substrate MBP that incubate at room temperature for 60 minutes. The specific activity is 42.52 nmol/min/mg.

Human

Sf9 insect cells

C-His

P28482-1 (M1-S360)

5594  [NCBI]

MAAAAAAGAGPEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYRS

Approximately 42 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液

Supplied as 0.22 μm filtered solution in 20 mM Tris, 500 mM Nacl, 10% glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.