1. 重组蛋白
  2. Cytokines and Growth Factors
  3. HB-EGF 蛋白, Mouse

HB-EGF 蛋白, Mouse

目录号: HY-P7194
COA 产品使用指南

HB-EGF 蛋白, Mouse在创伤愈合、心肌肥厚、心脏发育和功能等方面发挥重要作用。

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规格 价格 是否有货 数量
10 μg ¥900 In-stock
50 μg ¥2200 In-stock
100 μg   询价  

* Please select Quantity before adding items.

MCE 顾客使用本产品发表的科研文献

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

HB-EGF Protein, Mouse is shown to play a role in wound healing, cardiac hypertrophy, and heart development and function.

研究背景

肝素结合表皮生长因子样生长因子 (HBEGF) 是 EGF 家族的肝素结合成员。它是成纤维细胞和平滑肌细胞的有效有丝分裂原和趋化因子。与其他 EGF 家族成员一样,HB-EGF 结合并激活 EGF 受体 1 和 4。HB-EGF 已被证明能够以自分泌或旁分泌方式刺激多种细胞的生长,并参与基质增殖。HB-EGF 也是肿瘤生长和血管生成的有效诱导剂[1]

生物活性

The ED50 is <1 ng/mL as measured by murine Balb/c 3T3 cells, corresponding to a specific activity of >1.0 × 106IU/mg.

种属

Mouse

表达系统

E. coli

标签

Tag Free

蛋白编号

Q06186 (D63-L148)

基因 ID
蛋白结构
N-term
HB-EGF (D63-L148)
Accession # Q06186
C-term
中文名
重组小鼠肝素结合 EGF 样生长因子
同用名
rMuHB-EGF; Proheparin-binding EGF-like growth factor; HBEGF; DTR
氨基酸序列

DLEGTDLNLFKVAFSSKPQGLATPSKERNGKKKKKGKGLGKKRDPCLRKYKDYCIHGECRYLQEFRTPSCKCLPGYHGHRCHGLTL

分子量

Approximately 9.8 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized after extensive dialysis against 10 mM PB, 500 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

HB-EGF Protein, Mouse 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HY-P7194
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