1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interferon & Receptors
  4. IFN-alpha
  5. IFN-alpha 2a
  6. IFN-alpha 2/IFNA2 蛋白, Mouse (His)

IFN-alpha 2/IFNA2 蛋白, Mouse (His)

目录号: HY-P7544A
COA 产品使用指南

IFN-α 2/IFNA2 蛋白由巨噬细胞合成,具有有效的抗病毒活性。它与 IFNAR2 的相互作用在信号传导过程中发挥着关键作用,有助于形成复杂的抗病毒防御机制网络。IFN-alpha 2/IFNA2 蛋白, Mouse (His) 是重组的 IFN-α 2/IFNA2 蛋白,由 E. coli 表达,带有 N-6*His 标签。IFN-alpha 2/IFNA2 蛋白, Mouse (His) 全长 167 个氨基酸,分子量约为 ~20 kDa。

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     可免费申领三个不同产品的试用装。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

IFN-alpha 2/IFNA2 Protein, synthesized by macrophages, exhibits potent antiviral activities. Its interaction with IFNAR2 plays a pivotal role in signaling processes, contributing to the intricate network of antiviral defense mechanisms. IFN-alpha 2/IFNA2 Protein, Mouse (His) is the recombinant mouse-derived IFN-alpha 2/IFNA2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of IFN-alpha 2/IFNA2 Protein, Mouse (His) is 167 a.a., with molecular weight of ~20 kDa.

研究背景

IFN-α 2/IFNA2 主要由巨噬细胞合成,具有有效的抗病毒活性。通过与 IFNAR2 的相互作用,它参与关键的信号传导过程,为复杂的抗病毒防御机制网络做出贡献。

生物活性

Measured in a cytotoxicity assay using TF-1 human erythroleukemic cells. The ED50 this effect is 0.7778 ng/mL, corresponding to a specific activity is 1.286 × 106 units/mg.

  • Measured in a cytotoxicity assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1.914 ng/mL, corresponding to a specific activity is 5.225×105 units/mg.
种属

Mouse

表达系统

E. coli

标签

N-6*His

蛋白编号

P01573 (C24-E190)

基因 ID
蛋白结构
N-term
6*His
IFNA2 (C24-E190)
Accession # P01573
C-term
同用名
Interferon alpha 2; IFNA2 Protein, Mouse
氨基酸序列

CDLPHTYNLRNKRALKVLAQMRRLPFLSCLKDRQDFGFPLEKVDNQQIQKAQAIPVLRDLTQQTLNLFTSKASSAAWNATLLDSFCNDLHQQLNDLQTCLMQQVGVQEPPLTQEDALLAVRKYFHRITVYLREKKHSPCAWEVVRAEVWRALSSSVNLLPRLSEEKE

分子量

Approximately 20 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

IFN-alpha 2/IFNA2 Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IFN-alpha 2/IFNA2 Protein, Mouse (His)
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HY-P7544A
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