IL-6 蛋白, Rat (HEK293)

IL-6 Protein, Rat (HEK293) is a HEK293 cell derived multifunctional cytokine that can regulate various immune and inflammatory responses.

白介素 6 (IL-6) 是肝脏急性时相反应最重要的介质之一^[1]。白细胞介素 6 (IL-6) 在感染和组织损伤时迅速而短暂地产生，通过刺激急性期反应、造血和免疫反应来促进宿主防御。IL-6 促进幼稚 CD4⁺ T 细胞的特异性分化，从而在先天免疫反应与获得性免疫反应的联系中发挥重要作用。IL-6 与转化生长因子 (TGF)-β 结合，对于从幼稚 CD4⁺ T 细胞分化为 Th17 必不可少，但 IL-6 也会抑制 TGF-β 诱导的 Treg 分化^[2]。

1.The ED₅₀ is <3 pg/mL as measured by 7TD1 Cells.
2.Measured in a cell proliferation assay using TF-1 cells. The ED₅₀ for this effect is 16.72 pg/mL, corresponding to a specific activity is 5.981×10⁷ units/mg.

• 
  Measured in a cell proliferation assay using TF-1 cells. The ED₅₀ for this effect is 16.72 pg/mL, corresponding to a specific activity is 5.981×10⁷ units/mg.

Rat

HEK293

Tag Free

P20607 (F25-T211)

24498  [NCBI]

FPTSQVRRGDFTEDTTHNRPVYTTSQVGGLITYVLREILEMRKELCNGNSDCMNSDDALSENNLKLPEIQRNDGCFQTGYNQEICLLKICSGLLEFRFYLEFVKNNLQDNKKDKARVIQSNTETLVHIFKQEIKDSYKIVLPTPTSNALLMEKLESQKEWLRTKTIQLILKALEEFLKVTMRSTRQT

Approximately 19-26 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Frorath B, et al. Production of recombinant rat interleukin-6 in Escherichia coli using a novel highly efficient expression vector pGEX-3T. Send to  [Content Brief]

  [2]. Tanaka T, et al. IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol. 2014 Sep 4;6(10):a016295.  [Content Brief]