PNGase F 蛋白, F. meningosepticum (His)

The PNGase F protein acts as an enzyme catalyst that specifically cleaves entire glycans from glycoproteins. This process depends on the replacement of the glycosylated asparagine moiety by a polypeptide chain at both its amino (R1) and carboxyl (R2) termini, as shown in Reaction 1. PNGase F Protein, F. meningosepticum (His) is the recombinant PNGase F protein, expressed by E. coli , with N-His labeled tag. The total length of PNGase F Protein, F. meningosepticum (His) is 314 a.a., with molecular weight of ~35 kDa.

PNGase F（肽：N-糖苷酶 F）是一种蛋白质，具有从糖蛋白中裂解整个聚糖的卓越能力。该酶促过程涉及去除糖基化天冬酰胺部分，特别需要用多肽链取代天冬酰胺的氨基 (R1) 和羧基 (R2) 末端。PNGase F 通过促进 N 连接聚糖的释放，在糖蛋白的分析和操作中发挥着关键作用。这种酶活性对于蛋白质糖基化研究中的各种应用至关重要，使研究人员能够研究聚糖修饰对蛋白质的结构和功能影响（参考：未提供具体参考）。

Measured by its ability to deglycosylate ribonuclease B under denatured conditions. >50% ribonuclease B (10 μg) is deglycosylated by 2.4 ng rFmPNGase F within 60 minutes, as measured under the described conditions.

Others

E. coli

N-His

P21163 (A41-N354)

/

APADNTVNIKTFDKVKNAFGDGLSQSAEGTFTFPADVTTVKTIKMFIKNECPNKTCDEWDRYANVYVKNKTTGEWYEIGRFITPYWVGTEKLPRGLEIDVTDFKSLLSGNTELKIYTETWLAKGREYSVDFDIVYGTPDYKYSAVVPVIQYNKSSIDGVPYGKAHTLGLKKNIQLPTNTEKAYLRTTISGWGHAKPYDAGSRGCAEWCFRTHTIAINNANTFQHQLGALGCSANPINNQSPGNWAPDRAGWCPGMAVPTRIDVLNNSLTGSTFSYEYKFQSWTNNGTNGDAFYAISSFVIAKSNTPISAPVVTN

Approximately 35 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.