TREM-2 蛋白, Human (HEK293, His)

TREM-2 Protein is a receptor for amyloid beta protein 42, lipoprotein particles, and apolipoprotein. TREM-2 Protein is involved in cell proliferation and apoptosis through Wnt/β, MTOR, PI3K and NF-kappa-B signaling pathways. TREM-2 Protein is expressed by macrophages, immature monocyte-derived dendritic cells, osteoclasts, and microglia to activate the immune response. TREM-2 Protein, Human (HEK293, His) is the recombinant human-derived TREM-2 protein, expressed by HEK293 , with C-6*His labeled tag.

Triggering receptor expressed on myeloid cells 2 (TREM-2) 可与 TYROBP 形成受体信号复合物，介导配体结合后的信号传导和细胞活化。TREM-2 是淀粉样 β 蛋白 42 的受体并介导其被小胶质细胞摄取和降解。与淀粉样蛋白-β 42 结合介导小胶质细胞的活化、增殖、迁移、凋亡和促炎细胞因子如 IL6R、CCL3 和抗炎细胞因子 ARG1 的表达。TREM-2 是脂蛋白颗粒 (如 LDL、VLDL 和 HDL) 和载脂蛋白 (如 APOA1、APOA2、APOB、APOE、APOE2、APOE3、APOE4 和 CLU) 的受体，并增强它们在小胶质细胞中的摄取。TREM-2 作为 Wnt/β-连环蛋白信号级联的上游调节剂调节小胶质细胞增殖。TREM-2 通过激活 MTOR 对小胶质细胞的代谢起作用。TREM-2 抑制 PI3K 和 NF-kappa-B 信号对脂多糖的反应;从而促进吞噬，抑制促炎细胞因子和一氧化氮的产生，抑制细胞凋亡，增加 IL10和 TGFB 的表达。在氧化应激过程中，它促进抗凋亡 NF-kappa-B 信号传导和 ERK 信号传导。TREM-2 可触发巨噬细胞和树突状细胞的免疫反应激活介导细胞因子诱导的巨噬细胞融合形成的多核巨细胞，在树突状细胞中，可介导趋化因子受体 CCR7 的上调和树突状细胞的成熟和存活。

Immobilized Human TREM-2, at 1 μg/mL (100 μL/well) can bind Anti-TREM2 Antibody. The ED₅₀ is 30.5 ng/mL, corresponding to a specific activity is 3.28×10^4 units/mg.

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  Immobilized Human TREM-2, at 1 μg/mL (100 μL/well) can bind Anti-TREM2 Antibody,  The ED₅₀ for this effect is 30.5 ng/mL, corresponding to a specific activity is 3.28×10⁴units/mg.

Human

HEK293

C-6*His

Q9NZC2-1 (H19-S174)

54209  [NCBI]

HNTTVFQGVAGQSLQVSCPYDSMKHWGRRKAWCRQLGEKGPCQRVVSTHNLWLLSFLRRWNGSTAITDDTLGGTLTITLRNLQPHDAGLYQCQSLHGSEADTLRKVLVEVLADPLDHRDAGDLWFPGESESFEDAHVEHSISRSLLEGEIPFPPTS

22-40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.