1. Academic Validation
  2. Cyclic pulsation stress promotes bone formation of tissue engineered laminae through the F-actin/YAP-1/β-Catenin signaling axis

Cyclic pulsation stress promotes bone formation of tissue engineered laminae through the F-actin/YAP-1/β-Catenin signaling axis

  • NPJ Regen Med. 2021 Sep 6;6(1):51. doi: 10.1038/s41536-021-00164-w.
Linli Li  # 1 Hailong Li  # 1 Yiqun He 1 Han Tang 1 Jian Dong 1 Xujun Chen 1 Feizhou Lyu 2 3 Youhai Dong 4
Affiliations

Affiliations

  • 1 Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, Shanghai, China.
  • 2 Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, Shanghai, China. lufeizhou@hotmail.com.
  • 3 Department of Orthopedics, Huashan Hospital, Fudan University, Shanghai, China. lufeizhou@hotmail.com.
  • 4 Department of Orthopedics, Shanghai Fifth People's Hospital, Fudan University, Shanghai, China. youhaidong1964@163.com.
  • # Contributed equally.
Abstract

Mechanical loads are fundamental regulators of bone formation and remodeling. However, the molecular regulation of mechanotransduction during vertebral laminae regeneration remains poorly understood. Here, we found that cerebrospinal fluid pulsation (CSFP) stress-cyclic pulsation stress-could promote the osteogenic and angiogenic abilities of rat mesenchymal stromal cells (MSC), thereby promoting tissue-engineered laminae's bone and blood vessel formation. In the process, F-actin relayed CSFP stress to promote the nuclear translocation of YAP1, which then decreased the degradation and promoted the nuclear translocation of β-catenin. In turn, the nuclear translocation of β-catenin promoted the osteogenic differentiation and angiogenic abilities of MSC, thereby promoting tissue-engineered laminae's bone and blood vessel formation. Thus, we conclude that CSFP promotes the osteogenesis and angiogenesis of tissue-engineered laminae through the F-actin/YAP-1/β-catenin signaling axis. This study advances our understanding of vertebral laminae regeneration and provides potential therapeutic approaches for spinal degeneration after spinal laminectomy.

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