2. MAPK/ERK Pathway Apoptosis Cell Cycle/DNA Damage
  3. Mixed Lineage Kinase RIP kinase CDK
  4. NecroIr1

NecroIr1 是一种铱 (III) 复合物，是顺铂 (Cisplatin, HY-17394) 耐药肺癌细胞 (A549R) 的坏死诱导剂。NecroIr1 选择性积累在线粒体中，导致氧化应激和线粒体膜电位 (MMP) 的损失。NecroIr1 能够激活受体相互作用的丝氨酸苏氨酸激酶 3 (RIPK3) 和混合谱系激酶结构域样假激酶 (MLKL)，调节 CDK4 表达。

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NecroIr1 Chemical Structure

NecroIr1 Chemical Structure

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥7250
In-stock
1 mg ￥2045
In-stock
5 mg ￥4500
In-stock
10 mg ￥8000
In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

NecroIr1 相关抗体

Top Publications Citing Use of Products

查看 CDK 亚型特异性产品:

查看所有亚型
CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

NecroIr1 is an iridium(III) complex, serves as necroptosis inducers in Cisplatin (HY-17394)-resistant lung cancer cells (A549R). NecroIr1 selectively accumulates in mitochondria, leading to oxidative stress and loss of mitochondrial membrane potential (MMP). NecroIr1 activates receptor-interacting serine-threonine kinase 3 (RIPK3) and Mixed Lineage Kinase (MLKL), and regulates CDK4 expression[1].

IC50 & Target[1]

CDK4

 

RIPK3

 

RIPK1

 

体外研究
(In Vitro)

NecroIr1 (2 μM; 1-2 d) exerts subcellular distribution over 90% accumulated in mitochondria in non-labelled A549R and CFSElabelled L02 cells[1].
NecroIr1 (1.5 μM and 3 μM; 24 h) results ROS generation increasing and loss of mitochondrial membrane potential[1].
NecroIr1 (1.5 μM and 3 μM; 24 h) activates necroptosis proteins, increases the phosphorylation of RIPK1 and RIPK3[1].
NecroIr1 (0.75 μM and 1.5 μM; 24 h) induces necroptosis by arresting cell cycle at G0/G1[1].
NecroIr1 (0.75-3.0 μM; 24 h) inhibits A549R cells proliferation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

NecroIr1 相关抗体:

Western Blot Analysis[1]

Cell Line: A549R cells
Concentration: 1.5 μM and 3 μM
Incubation Time: 24 hours
Result: Increased phospho-RIPK1 (p-PIPK1), total RIPK3, and phospho-RIPK3 (p-PIPK3) level.

Cell Cycle Analysis[1]

Cell Line: A549R cells
Concentration: 0 μM, 0.75 μM and 1.5 μM
Incubation Time: 24 hours
Result: Arrested cell cycle at G0/G1 phase in a dose-dependent manner.

Cell Proliferation Assay[1]

Cell Line: A549R cells
Concentration: 0 μM, 0.75 μM, 1.5 μM and 3.0 μM
Incubation Time: 24 hours
Result: Inhibits cell proliferation in a dose-dependent manner.
分子量

823.36

Formula

C40H29ClIrN5O-
性状

固体

颜色

Orange to red

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : 100 mg/mL (121.45 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2145 mL 6.0727 mL 12.1454 mL
5 mM 0.2429 mL 1.2145 mL 2.4291 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

NecroIr1 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2145 mL 6.0727 mL 12.1454 mL 30.3634 mL
5 mM 0.2429 mL 1.2145 mL 2.4291 mL 6.0727 mL
10 mM 0.1215 mL 0.6073 mL 1.2145 mL 3.0363 mL
15 mM 0.0810 mL 0.4048 mL 0.8097 mL 2.0242 mL
20 mM 0.0607 mL 0.3036 mL 0.6073 mL 1.5182 mL
25 mM 0.0486 mL 0.2429 mL 0.4858 mL 1.2145 mL
30 mM 0.0405 mL 0.2024 mL 0.4048 mL 1.0121 mL
40 mM 0.0304 mL 0.1518 mL 0.3036 mL 0.7591 mL
50 mM 0.0243 mL 0.1215 mL 0.2429 mL 0.6073 mL
60 mM 0.0202 mL 0.1012 mL 0.2024 mL 0.5061 mL
80 mM 0.0152 mL 0.0759 mL 0.1518 mL 0.3795 mL
100 mM 0.0121 mL 0.0607 mL 0.1215 mL 0.3036 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NecroIr1NecroIr 1NecroIr-1Mixed Lineage KinaseRIP kinaseCDKMLKsReceptor-interacting protein kinasesRIPKCyclin dependent kinaseCisplatinCisplatin-resistant lung cancer cellsmitochondriamitochondrial membrane potentialMMPnecroptosisInhibitorinhibitorinhibit

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产品名称：
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目录号：
HY-148365
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