Progesterone (Synonyms: 黄体酮; Pregn-4-ene-3,20-dione)

目录号: HY-N0437 纯度: 99.79%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Progesterone是一种类固醇激素，可调节月经周期，对怀孕很重要。

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Progesterone Chemical Structure

CAS No. : 57-83-0

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥500	In-stock
100 mg	￥350	In-stock
1 g	￥550	In-stock
5 g	￥900	In-stock
10 g		询价
50 g		询价

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Customer Review

Other Forms of Progesterone:

MCE 顾客使用本产品发表的 22 篇科研文献

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生物活性
纯度 & 产品资料
参考文献

生物活性

Progesterone is a steroid hormone that regulates the menstrual cycle and is crucial for pregnancy.

IC₅₀ & Target

Human Endogenous Metabolite

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
Breast cancer cell line	EC₅₀	18.7 μM Compound: Progesterone	Concentration that reduces difference in reversal of [3H]VBL accumulation between MDA-435/LCC6 and MDA-435/LCC6-MDRI cells by 50% Concentration that reduces difference in reversal of [3H]VBL accumulation between MDA-435/LCC6 and MDA-435/LCC6-MDRI cells by 50%	[PMID: 11784143]
Breast cancer cell line	EC₅₀	22.3 μM Compound: Progesterone	Concentration that reduces the difference in reversal of DOX accumulation between MDA-435/LCC6 and MDA-435/LCC6-MDRI cells by 50% Concentration that reduces the difference in reversal of DOX accumulation between MDA-435/LCC6 and MDA-435/LCC6-MDRI cells by 50%	[PMID: 11784143]
CHO	EC₅₀	2.77 μM Compound: 44	Agonist activity at human TGR5 expressed in CHO cells by luciferase assay Agonist activity at human TGR5 expressed in CHO cells by luciferase assay	[PMID: 18307294]
CV-1	IC₅₀	> 10000 nM Compound: Progesterone	Antagonist activity at human progesterone receptor B expressed in african green monkey CV1 cells co-transfected with MMTV-Luc assessed as effect on progesterone-induced activity by luciferase reporter gene assay Antagonist activity at human progesterone receptor B expressed in african green monkey CV1 cells co-transfected with MMTV-Luc assessed as effect on progesterone-induced activity by luciferase reporter gene assay	[PMID: 20510622]
CV-1	EC₅₀	15.2 nM Compound: Progesterone	Agonist activity at human progesterone receptor B expressed in african green monkey CV1 cells co-transfected with MMTV-Luc after 24 hrs by luciferase reporter gene assay Agonist activity at human progesterone receptor B expressed in african green monkey CV1 cells co-transfected with MMTV-Luc after 24 hrs by luciferase reporter gene assay	[PMID: 20510622]
CV-1	EC₅₀	2.9 nM Compound: Progesterone	Effective concentration for agonist activity towards human progesterone receptor (hPR) using the cotransfection assay in CV-1 cells Effective concentration for agonist activity towards human progesterone receptor (hPR) using the cotransfection assay in CV-1 cells	[PMID: 12781197]
CV-1	EC₅₀	2.9 nM Compound: prog	Compound was tested for agonistic activity by cotransfection assay against human Progesterone receptor in CV-1 cells Compound was tested for agonistic activity by cotransfection assay against human Progesterone receptor in CV-1 cells	[PMID: 10212133]
HEK293	EC₅₀	2 nM Compound: prog	Agonist activity at human PRGR expressed in HEK293 cells by luciferase reporter gene assay Agonist activity at human PRGR expressed in HEK293 cells by luciferase reporter gene assay	[PMID: 25305688]
HeLa	IC₅₀	> 50 μg/mL Compound: 4	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 24021891]
K562/R7	IC₅₀	10.6 μM Compound: progesterone	Potentiation of doxorubicin-induced cytotoxicity against doxorubicin-resistant human K562/R7 cells assessed as doxorubicin IC50 at 1 uM after 72 hrs by MTT assay Potentiation of doxorubicin-induced cytotoxicity against doxorubicin-resistant human K562/R7 cells assessed as doxorubicin IC50 at 1 uM after 72 hrs by MTT assay	[PMID: 25634041]
LNCaP	IC₅₀	13.7 nM Compound: Progesterone	Competitive displacement of [3H]R1881 from human AR-LBD expressed in LNCaP cells incubated for 24 hrs by scintillation counting method based radioligand competitive binding assay Competitive displacement of [3H]R1881 from human AR-LBD expressed in LNCaP cells incubated for 24 hrs by scintillation counting method based radioligand competitive binding assay	[PMID: 30925341]
T47D	EC₅₀	0.1 nM Compound: P4	Activation of progesterone receptor in human T47D cells after 20 hrs by PRE-tagged luciferase reporter gene assay Activation of progesterone receptor in human T47D cells after 20 hrs by PRE-tagged luciferase reporter gene assay	[PMID: 19863083]
T47D	EC₅₀	0.92 nM Compound: P4	Agonistic activity against progesterone receptor in alkaline phosphatase assay using human T47D breast carcinoma cell line Agonistic activity against progesterone receptor in alkaline phosphatase assay using human T47D breast carcinoma cell line	[PMID: 11859003]
T47D	EC₅₀	1.8 nM Compound: prog	Agonistic activity by cotransfection assay against human Progesterone receptor in T47D cells Agonistic activity by cotransfection assay against human Progesterone receptor in T47D cells	[PMID: 10212133]

体内研究
(In Vivo)

Progesterone 可用于构建黄褐斑模型^[6]。

诱导黄褐斑^[6]

致病原理

Progesterone会增加色素沉着和体重。体重增加被认为是由于钠和液体潴留，这可能会进一步影响合成黑色素的黑素体的细胞内pH 值，进而导致黑色素的产生，因为酪氨酸酶活性与细胞内 pH 环境有关^[7]。

具体造模方法：

小鼠：6-8 周龄 • 雌性 • C57BL/6J 小鼠
给药方式：15 mg/kg • 每天肌肉注射，连续 30 天

Note

选取小鼠背部 3*3 cm²，剃光毛发后，用 UVB 照射（λ=312 nm，2 小时/天）。

造模成功指标

表型：引起皮肤组织损伤，结痂，皮肤变黑，表皮严重增生，毛囊坏死。
组织：纤维组织增生，表皮黑色素细胞富集。
分子：p-JNK /JNK、p-P38MAPK/P38MAPK 表达 •。

相关产品： /

拮抗产品： /

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Adult female mice (7-13 wk, 18-28 g)^[4]
Dosage:	1 mg
Administration:	Injections; three consecutive daily
Result:	Stimulated vessel maturation in the mouse endometrium.

Clinical Trial

分子量

314.46

Formula

C₂₁H₃₀O₂

CAS 号

57-83-0

性状

固体

颜色

White to off-white

中文名称

黄体酮；孕酮

初始来源

微生物

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : 50 mg/mL (159.00 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.1801 mL	15.9003 mL	31.8005 mL
5 mM	0.6360 mL	3.1801 mL	6.3601 mL
10 mM	0.3180 mL	1.5900 mL	3.1801 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.61 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.61 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.61 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： Corn Oil
Solubility: 10 mg/mL (31.80 mM); 澄清溶液; 超声助溶
方案二

请依序添加每种溶剂： 50% PEG300 50% Saline
Solubility: 20 mg/mL (63.60 mM); 悬浊液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.79%

选择批次：

Data Sheet (655 KB) SDS (418 KB)

COA (290 KB) HNMR (273 KB) RP-HPLC (245 KB) LCMS (93 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Schindler AE, et al. Classification and pharmacology of progestins. Maturitas. 2003 Dec 10;46 Suppl 1:S7-S16. [Content Brief]

[2]. Zava DT, et al. Estrogen and progestin bioactivity of foods, herbs, and spices. Proc Soc Exp Biol Med. 1998 Mar;217(3):369-78. [Content Brief]

[3]. Komesaroff PA, et al. Effects of wild yam extract on menopausal symptoms, lipids and sex hormones in healthy menopausal women. Climacteric. 2001 Jun;4(2):144-50. [Content Brief]

[4]. Girling JE, et al. Progesterone, but not estrogen, stimulates vessel maturation in the mouse endometrium. Endocrinology. 2007 Nov;148(11):5433-41. Epub 2007 Aug 9. [Content Brief]

[5]. Wong R, et al. Progesterone pharmacokinetics in the mouse: implications for potential stroke therapy. J Pharm Pharmacol. 2012 Nov;64(11):1614-20. [Content Brief]

[6]. Wang JY, et al. Licorice zinc suppresses melanogenesis via inhibiting the activation of P38MAPK and JNK signaling pathway in C57BL/6J mice skin. Acta Cir Bras. 2022 Dec 19;37(10):e371002. [Content Brief]

[7]. Hisayoshi NoRIMOTO, et al. Effects of keishibukuryoganryokayokuinin (gui-zhi-fu-ling-wanliao-jia-yiyiren) on the Epidermal Pigment Cells from DBA/2 Mice Exposed to Ultraviolet B (UVB) and/or Progesterone. The pharmaceutical Society of Japan. 2011, 131(11):1613-1619.

Progesterone 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1801 mL	15.9003 mL	31.8005 mL	79.5014 mL
	5 mM	0.6360 mL	3.1801 mL	6.3601 mL	15.9003 mL
	10 mM	0.3180 mL	1.5900 mL	3.1801 mL	7.9501 mL
	15 mM	0.2120 mL	1.0600 mL	2.1200 mL	5.3001 mL
	20 mM	0.1590 mL	0.7950 mL	1.5900 mL	3.9751 mL
	25 mM	0.1272 mL	0.6360 mL	1.2720 mL	3.1801 mL
	30 mM	0.1060 mL	0.5300 mL	1.0600 mL	2.6500 mL
	40 mM	0.0795 mL	0.3975 mL	0.7950 mL	1.9875 mL
	50 mM	0.0636 mL	0.3180 mL	0.6360 mL	1.5900 mL
	60 mM	0.0530 mL	0.2650 mL	0.5300 mL	1.3250 mL
	80 mM	0.0398 mL	0.1988 mL	0.3975 mL	0.9938 mL
	100 mM	0.0318 mL	0.1590 mL	0.3180 mL	0.7950 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Progesterone57-83-0Pregn-4-ene-3,20-dione黄体酮孕酮Progesterone ReceptorEndogenous MetaboliteNR3C3Inhibitorinhibitorinhibit

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Progesterone (Synonyms: 黄体酮; Pregn-4-ene-3,20-dione)

Customer Review

Other Forms of Progesterone:

MCE 顾客使用本产品发表的 22 篇科研文献

Progesterone 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

生物活性

纯度 & 产品资料

参考文献

摩尔计算器

稀释计算器

Progesterone 相关分类

完整储备液配制表

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Progesterone (Synonyms: 黄体酮; Pregn-4-ene-3,20-dione)

Customer Review

Other Forms of Progesterone:

Progesterone 相关抗体

MCE 顾客使用本产品发表的 22 篇科研文献

Progesterone 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

生物活性

纯度 & 产品资料

参考文献

摩尔计算器

稀释计算器

Progesterone 相关分类

完整储备液配制表

Keywords:

您最近查看的产品:

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