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细胞自噬检测

细胞自噬检测是用于评估细胞自噬过程的技术。这包括检测自噬小体、自噬相关蛋白的表达及其活性。常见方法包括光学显微镜下观察、Western blot、荧光染色等。自噬是一种细胞自身分解和回收细胞器及蛋白的重要生理过程,参与调节细胞生存与死亡。自噬检测在疾病研究、药物筛选和生物医学研究中有广泛应用。


Autophagy assays are techniques used to evaluate the autophagy process. This includes detecting the expression of autophagosomes, autophagy-related proteins, and their activities. Common methods include observation under a light microscope, Western blot, fluorescent staining, etc. Autophagy is an important physiological process in which cells decompose and recycle organelles and proteins. It is involved in regulating cell survival and death. Autophagy assays are widely used in disease research, drug screening, and biomedical research.

相关实验方案

  • 自噬是真核细胞在自噬相关基因的调控下利用溶酶体降解自身细胞质蛋白和受损细胞器的过程。微管相关蛋白轻链3 (LC3)是目前公认的自噬标记物,在自噬过程中由胞浆型 (LC3-I) 被酶为膜型 (LC3-II),可通过Western Blot 和荧光显微镜可检测LC3-II/I 比值。