CCL22/MDC 蛋白, Human

CCL22/MDC Protein, Human is a CC chemokine that acts as a ligand for the CCR4 receptor and is a chemoattractant for CCR4-expressing cells such as Th2 cells, playing an important role in homeostatic and inflammatory responses. CCL22/MDC Protein, Human is a recombinant human CCL22/MDC (G25-Q93) protein expressed by E. coli^[1][2].

CCL22又称巨噬细胞源性趋化因子（MDC），是一种位于人类基因组16号染色体上的CC趋化因子，是由CCL22基因编码的蛋白质，在氨基酸水平上与CCL17有37%的同一性。CCL22由树突状细胞和巨噬细胞分泌，可被脂多糖、细胞因子等多种刺激因子上调。其中，Th2细胞因子IL-4和IL-13诱导骨髓细胞产生CCL22，并可被Th1细胞因子IFN-γ抑制。除了作为表达 CCR4 的 Th2 淋巴细胞、单核细胞、单核细胞衍生的树突细胞和自然杀伤细胞的有效趋化剂外，CCL22 还可以通过与趋化因子受体 CCR4 相互作用来影响其靶细胞。CCL22是CCR4内化的有效诱导剂，CCL22与CCR4的结合降低了CCR4随后的功能反应。CCL22 与 CCR4 的相互作用涉及多种病理学，从过敏反应、自身免疫到肿瘤生长。相比之下，能够阻断 CCL17 和 CCL22 介导的 Th2 和 Treg 细胞募集的小分子化合物和抗体已被证明在哮喘、特应性疾病和肿瘤生长的各种疾病模型中具有积极作用。此外，CCL22 及其受体在 TH2 淋巴细胞募集中的重要作用已在过敏性气道炎症模型中得到证实。它还可以通过调节成熟胸腺细胞通过该器官的迁移来参与胸腺生成^[1][2]。

CCL22/MDC (100 ng/mL, 24 h) treatment alone dose not result in increased production of chemokine IL-8, and treatment of hBEC with TNF-α increased IL-8 secretion. In addition, treatment of hBEC with both TNF-α and MDC results in increased IL-8 production than treatment with TNF-α alone in human bronchial epithelial cells (hBEC)^[3].

1.The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/mL.
2.Measured by its ability to chemoattract Jurkat cells. The ED₅₀ for this effect is ≤43.72 ng/mL, corresponding to a specific activity is ≥2.287×10⁴ U/mg.

• 
  Measured by its ability to chemoattract Jurkat cells. The ED₅₀ for this effect is 43.72 ng/mL, corresponding to a specific activity is 2.287×10⁴ U/mg.

Human

E. coli

Tag Free

O00626 (G25-Q93)

6367  [NCBI]

GPYGANMEDSVCCRDYVRYRLPLRVVKHFYWTSDSCPRPGVVLLTFRDKEICADPRVPWVKMILNKLSQ

Approximately 8.1-9 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, pH 7.4 or PBS, pH 7.4.

<1 EU/μg; determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Jan Korbecki, et al. CC Chemokines in a Tumor: A Review of Pro-Cancer and Anti-Cancer Properties of the Ligands of Receptors CCR1, CCR2, CCR3, and CCR4. Int J Mol Sci. 2020 Nov 9;21(21):8412.  [Content Brief]

  [2]. Stefanie Scheu, et al. The C-C Chemokines CCL17 and CCL22 and Their Receptor CCR4 in CNS Autoimmunity. Int J Mol Sci. 2017 Nov 2;18(11):2306.  [Content Brief]

  [3]. Jillian R Richter, et al. Macrophage-derived chemokine (CCL22) is a novel mediator of lung inflammation following hemorrhage and resuscitation. Shock. 2014 Dec;42(6):525-31.  [Content Brief]