CD47 蛋白, Mouse (HEK293, Tag Free)

The CD47 protein, also known as integrin-associated protein (IAP), is an immune checkpoint protein. CD47 binds to membrane integrins and binds ligands platelet reaction-protein-1 (TSP-1) and signal regulatory protein alpha (SIRP伪). CD47 is a potential therapeutic target for some cancers and is also used to treat pulmonary fibrosis. CD47 Protein, Mouse (HEK293, Tag Free) is the recombinant mouse-derived CD47 protein, expressed by HEK293 , with tag free. The total length of CD47 Protein, Mouse (HEK293, Tag Free) is 122 a.a., with molecular weight of ~27-34 kDa.

CD47 也被称为整合素相关蛋白 (IAP)，是一种由 CD47 基因编码的跨膜蛋白。CD47 属于免疫球蛋白超家族，与膜整合素结合，并结合配体血小板反应蛋白-1 (TSP-1) 和信号调节蛋白 α (SIRPα)。CD47 参与多种生物过程，包括信号转导、心血管稳态、炎症、凋亡、血管生成、细胞自我更新和免疫调节。此外，CD47 在海马的记忆形成和突触可塑性中也很重要。它作为 SIRPA 的受体，阻止未成熟树突状细胞成熟，抑制成熟树突状细胞产生细胞因子。与 SIRPG 的相互作用增强细胞-细胞粘附、T 细胞增殖和 T 细胞活化。CD47 正调控 FAS 依赖性 T 细胞凋亡并抑制血管生成。它还可能参与正常衰老过程中的代谢失调，调节伤口愈合和干细胞自我更新。CD47 可能在细胞膜运输、整合素依赖的信号转导和预防红细胞过早清除中发挥作用。它与 THBS1、SIRPA、FAS/CD95、SIRPG、UBQLN1、UBQLN2 和纤维蛋白原相互作用。CD47 受体的激活可通过 AKT 依赖性途径引起人星形细胞瘤细胞的增殖。CD47 是某些癌症的潜在治疗靶点，也用于治疗肺纤维化。

Measured by its binding ability in a functional ELISA. Immobilized Mouse CD47 at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse SIRP alpha. The ED₅₀ for this effect is 1.14 μg/mL, corresponding to a specific activity is 877.2 Unit/mg.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Mouse CD47 at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse SIRP alpha. The ED₅₀ for this effect is 1.14 μg/mL, corresponding to a specific activity is 877.2 Unit/mg.

Mouse

HEK293

Tag Free

ADQ12919.1 (Q19-K140)

16423

QLLFSNVNSIEFTSCNETVVIPCIVRNVEAQSTEEMFVKWKLNKSYIFIYDGNKNSTTTDQNFTSAKISVSDLINGIASLKMDKRDAMVGNYTCEVTELSREGKTVIELKNRTVSWFSPNEK

Approximately 27-34 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.