PDGF-CC 蛋白, Cynomolgus (HEK293, hFc)

Platelet derived growth factor C (PDGF-CC) is a growth factor that plays an essential role in the regulation of cell proliferation, cell migration, survival and chemotaxis. PDGF-CC is a potent mitogen and chemoattractant for cells of mesenchymal origin which plays an important roll in normal skeleton formation and normal skin morphogenesis during embryonic development, wound healing, angiogenesis and blood vessel development as well as being involved in fibrotic processes. PDGF-CC Protein, Cynomolgus (HEK293, hFc) is the recombinant cynomolgus-derived PDGF-CC protein, expressed by HEK293 , with N-hFc labeled tag. The total length of PDGF-CC Protein, Cynomolgus (HEK293, hFc) is 111 a.a., with molecular weight of ~45 kDa.

血小板衍生生长因子 C (PDGF-CC) 是一种重要的生长因子，在胚胎发育、细胞增殖、细胞迁移、存活和趋化过程中发挥重要作用。PDGF-CC 是一种有效的间充质细胞有丝分裂原和化学引诱剂，这是胚胎发育过程中正常骨骼形成所必需的，特别是颅面骨骼的正常发育和腭的正常发育。PDGF-CC 也是胚胎发育过程中正常皮肤形态发生所必需的。PDGF-CC 在伤口愈合中起着重要作用，它似乎参与了三个阶段:炎症、增殖和重塑。PDGF-CC 在血管生成和血管发育中起重要作用，并参与纤维化过程，其中间质成纤维细胞转化为肌成纤维细胞并发生胶原沉积。PDGF-CC 的 CUB 结构域在冠状动脉平滑肌细胞中具有有丝分裂活性^[1][2][3]。

Measured in a cell proliferation assay using NIH/3T3 cells. The ED₅₀ for this effect is 15.09 ng/mL, corresponding to a specific activity is 66269.052 units/mg.

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  Measured in a cell proliferation assay using NIH/3T3 cells. The ED₅₀ for this effect is 15.09 ng/mL, corresponding to a specific activity is 66269.052 units/mg.

Cynomolgus

HEK293

N-hFc

EHH54037 (V196-G306)

/

VVDLNLLTEEVRLYSCTPRNFSVSIREELKRTDTIFWPGCLLVKRCGGNCACCLHNCNECQCVPSKVTKKYHEVLQLRPKTGVRGLHKSLTDVALEHHEECDCVCRGSTGG

Approximately 45 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.