Wnt3a Surrogate 蛋白, Human (HEK293, Fc)

Wnt3a Surrogate protein is a Wnt recombination protein that plays an important role in embryonic development, adult tissue homeostasis and injury repair. Wnt3a Surrogate protein, consisting of the domain and Fc portion of Wnt3a, helps organoids expand and maintain a source of Wnt. Organoids are a rapidly evolving model system for tissue development, and Wnt3a is considered as the essential protein for organoid growth. Wnt3a or the small molecule glycogen synthase kinase 3 (GSK3) inhibitor CHIR are commonly used as Wnt sources in experiments. However, the application of Wnt3a is hampered by Wnt lipidation, and cross-reactivity with Wnt frizzled (Fzd). Therefore, Wnt3a Surrogate protein was used instead of Wnt3a, as a water-soluble and Fzd-specific alternative Wnt agonist. And the Wnt3a Surrogate protein exhibits tissue selectivity consistent with Fzd isoform specificity^[1][2][3]. Wnt3a Surrogate Protein, Human (HEK293, Fc) is a recombinant Wnt3a protein of human origin. It is expressed by HEK293 cells with an Fc tag.

Wnt 是一个分泌型糖蛋白，包含 19 种人类蛋白质，通过经典通路和非经典通路发出信号。Wnt 参与了发育形态、干细胞维持和伤口愈合，并可长距离移动或附着在外泌体表面。其中 Wnt3a 是一种重要的分子，可以激活 Wnt/β-catenin 信号通路，参与多种生理和病理过程，如胚胎发育、组织修复以及肿瘤进展等。Wnt3a 还在心肌发生、骨骼肌发育、神经系统形成和滋养细胞的迁移和侵袭中发挥作用^[4][5]。
1.Wnt 家族包括：Wnt1、Wnt2、Wnt2b（Wnt13）、Wnt3、Wnt3a、Wnt4、Wnt5a、Wnt5b、Wnt6、Wnt7a、Wnt7b、Wnt8a、Wnt8b、Wnt9a（Wnt14）、Wnt9b（Wnt14b）、Wnt10a、 Wnt10b、Wnt11 和 Wnt16。
2.Wnt 经典途径：也称为 Wnt/β-catenin 途径。
3.Wnt 非经典通路：包括 Wnt-平面细胞极性通路（Wnt-PCP 通路）和 Wnt-钙通路（Wnt-Ca²⁺ 通路）。
4.Wnt3a 介绍：是细胞信号传导和发育过程中的重要分子，具有高度保守性。Wnt3a 与 Frizzled 受体相互作用，诱导 β-连环蛋白的积累，激活 Wnt/β-catenin 信号通路。Wnt3a 还能刺激滋养细胞的迁移和侵袭。
Wnt3a 功能：在胚胎发育中，Wnt3a 参与了胚胎轴的形成和定向细胞分化；在成人组织中，Wnt3a 与调节干细胞自我更新和细胞命运决定有关，同时也与某些肿瘤的发生和发展相关。例如 Wnt3a 通过典型的 Wnt 信号通路促进或抑制肿瘤进展。Wnt3a 也与受伤后重塑和细胞扩增相关，WNT3a 上调可改善修复。Wnt3a 在器官形成中也起着重要作用。它可以通过增加T细胞特异性转录因子 (TCF) 依赖性转录和诱导心脏特异性标记物、骨形态发生蛋白 (BMPs) 和肌球蛋白重链 (MHC) 来促进心肌发生。研究表明，Wnt3a 可诱导分化体肌组的肌生成，激活肌源性分化抗原 (MyoD) 表达，进而促进骨骼肌发育。Wnt3a 激活非典型蛋白激酶 Cloys (pKCiota) 并诱导 pKCiota 在神经突和细胞质中呈点状分布，在中心体处具有特别强烈的信号。Wnt3a 在神经中胚层干细胞的维持和分化中起着至关重要的作用。因此，Wnt3a 参与了神经系统的形成。

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED₅₀ for this effect is <62 ng/mL.

Human

HEK293

Fc

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66-72 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 25 mM Tris-HCl, 500 mM NaCl, pH 8.2.

< 0.01 EU/µg as determined by LAL test.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Janda CY, et al. Surrogate Wnt agonists that phenocopy canonical Wnt and β-catenin signalling. Nature. 2017 May 11;545(7653):234-237.  [Content Brief]

  [2]. Chen H, et al. Development of Potent, Selective Surrogate WNT Molecules and Their Application in Defining Frizzled Requirements. Cell Chem Biol. 2020 May 21;27(5):598-609.e4.  [Content Brief]

  [3]. Miao Y, et al. Next-Generation Surrogate Wnts Support Organoid Growth and Deconvolute Frizzled Pleiotropy In Vivo. Cell Stem Cell. 2020 Nov 5;27(5):840-851.e6.  [Content Brief]

  [4]. He S, et al. Wnt3a: functions and implications in cancer. Chin J Cancer. 2015 Sep 14;34(12):554-62.  [Content Brief]

  [5]. Thomas BL, et al. WNT3A-loaded exosomes enable cartilage repair. J Extracell Vesicles. 2021 May;10(7):e12088.  [Content Brief]