Retinoic acid (Synonyms: 维生素A酸; Vitamin A acid; all-trans-Retinoic acid; ATRA)

目录号: HY-14649 纯度: 99.81%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Retinoic acid 是维生素 A 的代谢产物，在细胞生长，分化和器官发生中起重要作用。Retinoic acid 是 RAR 核受体的天然激动剂，对 RARα/β/γ的 IC₅₀ 为14 nM。 Retinoic acid与 PPARβ/δ 结合的 K_d 值为 17 nM。Retinoic acid 可以通过激活视黄酸受体抑制转录因子 Nrf2。

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Retinoic acid Chemical Structure

CAS No. : 302-79-4

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥550	In-stock
100 mg	￥500	In-stock
500 mg	￥750	In-stock
1 g	￥1014	In-stock
5 g		询价
10 g		询价

or 大包装询价

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Customer Review

Other Forms of Retinoic acid:

MCE 顾客使用本产品发表的 62 篇科研文献

: Retinoic acid purchased from MCE. Usage Cited in: Oncotarget. 2017 Nov 22;8(65):109135-109150. [Abstract]; MCF-7 cells are pretreated with the indicated chemical inhibitors for 30min, followed by 15 min treatment with RA (20 μM) + EPA (80 μM).Cell extracts are prepared and subjected to western blotting analysis.

: Retinoic acid purchased from MCE. Usage Cited in: Oncotarget. 2017 Nov 22;8(65):109135-109150. [Abstract]; Cells are treated with RA (20 μM) plus ω-3 PUFAs (80 μM) with or without CQ (5 μM) for 24 h. Cell extracts are prepared and subjected to western blotting analysis.

: Retinoic acid purchased from MCE. Usage Cited in: Oncotarget. 2017 Nov 22;8(65):109135-109150. [Abstract]; Cell morphology of MCF-7 treated with RA (20 μM)+ω-3 PUFAs (80 μM) with or without 3-MA (5 mM) for 24h.

Retinoic acid 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 RAR/RXR 亚型特异性产品:

查看所有亚型

RXRα RXRβ RXRγ

查看 PPAR 亚型特异性产品:

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PPARα PPARβ/δ PPARγ PPAR PPARδ

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Retinoic acid is a metabolite of vitamin A that plays important roles in cell growth, differentiation, and organogenesis. Retinoic acid is a natural agonist of RAR nuclear receptors, with IC₅₀s of 14 nM for RARα/β/γ. Retinoic acid bind to PPARβ/δ with K_d of 17 nM. Retinoic acid acts as an inhibitor of transcription factor Nrf2 through activation of retinoic acid receptor alpha.

IC₅₀ & Target^[2]^[5]

PPARβ/δ

17 nM (Kd)

PPARα

103 nM (Kd)

PPARγ

178 nM (Kd)

Human Endogenous Metabolite

PPARα

14 nM (IC₅₀)

PPARγ

14 nM (IC₅₀)

RARβ

14 nM (IC₅₀)

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
3LLD122	IC₅₀	33 μM Compound: ATRA	Concentration required to inhibit the colony formation of lung carcinoma (3LLD122) cell lines by 50% Concentration required to inhibit the colony formation of lung carcinoma (3LLD122) cell lines by 50%	[PMID: 10956204]
COLO 205	IC₅₀	37 μM Compound: ATRA	Cytotoxicity against human COLO205 cells after 48 hrs by MTT assay Cytotoxicity against human COLO205 cells after 48 hrs by MTT assay	[PMID: 20405849]
COS-7	EC₅₀	0.2 nM Compound: ATRA	Activity at human RARgamma ligand binding domain expressed in COS7 cells co-transfected with Gal4-DBD assessed as transcriptional activation after 16 hrs by Gal4 response element-driven luciferase reporter gene assay Activity at human RARgamma ligand binding domain expressed in COS7 cells co-transfected with Gal4-DBD assessed as transcriptional activation after 16 hrs by Gal4 response element-driven luciferase reporter gene assay	[PMID: 19058965]
COS-7	EC₅₀	0.6 nM Compound: ATRA	Transactivation of GAL4-fused mouse RARgamma-LBD expressed in COS-7 cells after 24 hrs by bright-Glo reagent based assay Transactivation of GAL4-fused mouse RARgamma-LBD expressed in COS-7 cells after 24 hrs by bright-Glo reagent based assay	[PMID: 30792038]
COS-7	EC₅₀	1.2 nM Compound: ATRA	Transactivation of GAL4-fused mouse RARalpha-LBD expressed in COS-7 cells after 24 hrs by bright-Glo reagent based assay Transactivation of GAL4-fused mouse RARalpha-LBD expressed in COS-7 cells after 24 hrs by bright-Glo reagent based assay	[PMID: 30792038]
COS-7	EC₅₀	1.2 nM Compound: ATRA	Transactivation of GAL4-fused mouse RARbeta-LBD expressed in COS-7 cells after 24 hrs by bright-Glo reagent based assay Transactivation of GAL4-fused mouse RARbeta-LBD expressed in COS-7 cells after 24 hrs by bright-Glo reagent based assay	[PMID: 30792038]
COS-7	EC₅₀	12 nM Compound: ATRA	Activity at human RARbeta ligand binding domain expressed in COS7 cells co-transfected with Gal4-DBD assessed as transcriptional activation after 16 hrs by Gal4 response element-driven luciferase reporter gene assay Activity at human RARbeta ligand binding domain expressed in COS7 cells co-transfected with Gal4-DBD assessed as transcriptional activation after 16 hrs by Gal4 response element-driven luciferase reporter gene assay	[PMID: 19058965]
COS-7	EC₅₀	17 nM Compound: ATRA	Activity at human RARalpha ligand binding domain expressed in COS7 cells co-transfected with Gal4-DBD assessed as transcriptional activation after 16 hrs by Gal4 response element-driven luciferase reporter gene assay Activity at human RARalpha ligand binding domain expressed in COS7 cells co-transfected with Gal4-DBD assessed as transcriptional activation after 16 hrs by Gal4 response element-driven luciferase reporter gene assay	[PMID: 19058965]
CV-1	EC₅₀	0.7 nM Compound: RA	Compound was tested for functional activity in CV-1 cells transfected with an expression vector for retinoic acid receptor gamma using transactivation assay Compound was tested for functional activity in CV-1 cells transfected with an expression vector for retinoic acid receptor gamma using transactivation assay	10.1016/0960-894X(95)00588-K
CV-1	EC₅₀	1 nM Compound: RA	Compound was tested for functional activity in CV-1 cells transfected with an expression vector for retinoic acid receptor beta using transactivation assay Compound was tested for functional activity in CV-1 cells transfected with an expression vector for retinoic acid receptor beta using transactivation assay	10.1016/0960-894X(95)00588-K
CV-1	EC₅₀	1100 nM Compound: RA	Compound was tested for functional activity in CV-1 cells transfected with an expression vector for retinoid X receptor gamma using transactivation assay Compound was tested for functional activity in CV-1 cells transfected with an expression vector for retinoid X receptor gamma using transactivation assay	10.1016/0960-894X(95)00588-K
CV-1	EC₅₀	1400 nM Compound: RA	Compound was tested for functional activity in CV-1 cells transfected with an expression vector for retinoid X receptor beta using transactivation assay Compound was tested for functional activity in CV-1 cells transfected with an expression vector for retinoid X receptor beta using transactivation assay	10.1016/0960-894X(95)00588-K
CV-1	EC₅₀	7 nM Compound: RA	Compound was tested for functional activity in CV-1 cells transfected with an expression vector for retinoic acid receptor alpha using transactivation assay Compound was tested for functional activity in CV-1 cells transfected with an expression vector for retinoic acid receptor alpha using transactivation assay	10.1016/0960-894X(95)00588-K
CV-1	EC₅₀	900 nM Compound: RA	Compound was tested for functional activity in CV-1 cells transfected with an expression vector for retinoid X receptor alpha using transactivation assay Compound was tested for functional activity in CV-1 cells transfected with an expression vector for retinoid X receptor alpha using transactivation assay	10.1016/0960-894X(95)00588-K
CWR22R	GI₅₀	25.11 μM Compound: 1, ATRA	Growth inhibition of human CWR22Rv1 cells by MTT assay Growth inhibition of human CWR22Rv1 cells by MTT assay	[PMID: 25634130]
DU-145	GI₅₀	11.64 μM Compound: ATRA, Tretinoin	Cytotoxicity against human DU145 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human DU145 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 25701251]
F9	IC₅₀	0.1 nM Compound: 7, trans- RA	Induction of terminal differentiation in mouse F9 cells assessed by measuring secreted plasminogen activator activity after 3 days Induction of terminal differentiation in mouse F9 cells assessed by measuring secreted plasminogen activator activity after 3 days	[PMID: 17489579]
F9	ED₅₀	0.1 nM Compound: 1	Ability to displace 3 uM retinoid and [3H]all-trans-retinoic acid in F9 embryonal carcinoma cells using F9 Plasminogen Activator releasing assay Ability to displace 3 uM retinoid and [3H]all-trans-retinoic acid in F9 embryonal carcinoma cells using F9 Plasminogen Activator releasing assay	[PMID: 2738885]
Fibroblast	IC₅₀	10 μM Compound: retinoic acid	Inhibition of UVB irradiation-induced MMP1 production in human dermal fibroblasts after 48 hrs Inhibition of UVB irradiation-induced MMP1 production in human dermal fibroblasts after 48 hrs	[PMID: 18029185]
HEK-293T	IC₅₀	28.7 μM Compound: Retinoic acid	Inhibition of mouse Ido2 transfected in HEK293T cells using L-tryptophan as substrate assessed as kynurenine formation after 45 mins by spectrophotometric analysis Inhibition of mouse Ido2 transfected in HEK293T cells using L-tryptophan as substrate assessed as kynurenine formation after 45 mins by spectrophotometric analysis	[PMID: 23122865]
HEK-293T	IC₅₀	301.3 μM Compound: Retinoic acid	Inhibition of mouse Ido1 transfected in HEK293T cells using L-tryptophan as substrate assessed as kynurenine formation after 45 mins by spectrophotometric analysis Inhibition of mouse Ido1 transfected in HEK293T cells using L-tryptophan as substrate assessed as kynurenine formation after 45 mins by spectrophotometric analysis	[PMID: 23122865]
HL-60	ED₅₀	2.4 nM Compound: retinoic acid	The ability to induce differentiation of human promyelocytic leukemia cell line HL-60 to mature granulocytes was determined by nitro blue tetrazolium (NBT) reduction assay The ability to induce differentiation of human promyelocytic leukemia cell line HL-60 to mature granulocytes was determined by nitro blue tetrazolium (NBT) reduction assay	[PMID: 8182710]
HL-60	ED₅₀	2.4 nM Compound: RA	Differentiation inducing activity towards human promyelocytic leukemia cell line HL-60 assayed by nitroblue tetrazolium reduction Differentiation inducing activity towards human promyelocytic leukemia cell line HL-60 assayed by nitroblue tetrazolium reduction	[PMID: 2704028]
HL-60	CC₅₀	23 nM Compound: ATRA; RA	Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition measured after 72 hrs by trypan blue staining based assay Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition measured after 72 hrs by trypan blue staining based assay	[PMID: 33895500]
HL-60	ED₅₀	50 nM Compound: 1	Compound was evaluated for the retinoid-induced differentiation of the human myeloid leukemia cell line HL-60 using trans-retinoic acid as the standard. Compound was evaluated for the retinoid-induced differentiation of the human myeloid leukemia cell line HL-60 using trans-retinoic acid as the standard.	[PMID: 1992144]
HT-29	CC₅₀	4.3 μM Compound: ATRA; RA	Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human HT-29 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 33895500]
HT-29	IC₅₀	4.3 μM Compound: ATRA	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 20405849]
LNCaP	IC₅₀	10 μM Compound: 1	Growth inhibition of human LNCaP cells after 6 days by MTT assay Growth inhibition of human LNCaP cells after 6 days by MTT assay	[PMID: 15615521]
LNCaP	GI₅₀	10.33 μM Compound: ATRA, Tretinoin	Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 25701251]
LNCaP	GI₅₀	47.86 μM Compound: 1, ATRA	Growth inhibition of human LNCAP cells by MTT assay Growth inhibition of human LNCAP cells by MTT assay	[PMID: 25634130]
LNCaP	IC₅₀	5 x 10^-1 μM Compound: ATRA	Antiproliferative activity against human LNCAP cells after 48 hrs by MTT assay Antiproliferative activity against human LNCAP cells after 48 hrs by MTT assay	[PMID: 19375825]
MCF7	IC₅₀	0.58 μM Compound: 1	Growth inhibition of human MCF7 cells after 6 days by MTT assay Growth inhibition of human MCF7 cells after 6 days by MTT assay	[PMID: 15615521]
MCF7	GI₅₀	12.39 μM Compound: ATRA, Tretinoin	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 25701251]
MCF7	CC₅₀	20 nM Compound: ATRA; RA	Antiproliferative activity in human MCF7 cells assessed as cell growth inhibition measured after 72 hrs by cell counting assay Antiproliferative activity in human MCF7 cells assessed as cell growth inhibition measured after 72 hrs by cell counting assay	[PMID: 33895500]
MCF7	IC₅₀	6.14 μM Compound: atRA	Antiproliferative activity against human MCF7 cells assessed as cell viability after 24 hrs Antiproliferative activity against human MCF7 cells assessed as cell viability after 24 hrs	[PMID: 33139111]
MDA-MB-231	GI₅₀	10.85 μM Compound: ATRA	Growth inhibition of human MDA-MB-231 cells after 5 days by MTT assay Growth inhibition of human MDA-MB-231 cells after 5 days by MTT assay	[PMID: 18543902]
MDA-MB-231	GI₅₀	14.12 μM Compound: 1, ATRA	Growth inhibition of human MDA-MB-231 cells by MTT assay Growth inhibition of human MDA-MB-231 cells by MTT assay	[PMID: 25634130]
MDA-MB-231	IC₅₀	16 μM Compound: atRA	Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 24 hrs Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 24 hrs	[PMID: 33139111]
MDA-MB-453	GI₅₀	9.51 μM Compound: ATRA, Tretinoin	Cytotoxicity against human MDA-MB-453 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-453 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 25701251]
MDA-MB-468	GI₅₀	14.12 μM Compound: 1, ATRA	Growth inhibition of human MDA-MB-468 cells by MTT assay Growth inhibition of human MDA-MB-468 cells by MTT assay	[PMID: 25634130]
MIA PaCa-2	IC₅₀	9.5 μM Compound: ATRA	Concentration required to inhibit the colony formation of pancreatic human (PACA) cell lines by 50% Concentration required to inhibit the colony formation of pancreatic human (PACA) cell lines by 50%	[PMID: 10956204]
MOLM-13	CC₅₀	1.24 μM Compound: ATRA; RA	Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell growth measured after 96 hrs by WST-8 assay Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell growth measured after 96 hrs by WST-8 assay	[PMID: 33895500]
NB-4	CC₅₀	1.5 μM Compound: ATRA; RA	Antiproliferative activity against human NB-4 cells assessed as inhibition of cell growth measured after 96 hrs by MTT assay Antiproliferative activity against human NB-4 cells assessed as inhibition of cell growth measured after 96 hrs by MTT assay	[PMID: 33895500]
PC-3	GI₅₀	12.64 μM Compound: ATRA, Tretinoin	Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 25701251]
PC-3	CC₅₀	12.7 μM Compound: ATRA; RA	Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth measured after 24 hrs by MTT assay Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth measured after 24 hrs by MTT assay	[PMID: 33895500]
PC-3	IC₅₀	2 μM Compound: 1	Growth inhibition of human PC3 cells after 6 days by MTT assay Growth inhibition of human PC3 cells after 6 days by MTT assay	[PMID: 15615521]
PC-3	GI₅₀	36.3 μM Compound: 1, ATRA	Growth inhibition of human PC3 cells by MTT assay Growth inhibition of human PC3 cells by MTT assay	[PMID: 25634130]
PC-3	GI₅₀	7.6 μM Compound: ATRA	Growth inhibition of human PC3 cells after 5 days by MTT assay Growth inhibition of human PC3 cells after 5 days by MTT assay	[PMID: 18543902]
Raji	IC₅₀	15.4 nM Compound: retinoic acid	Inhibition of TPA-induced Epstein-Barr virus early antigen activation in Raji cells after 48 hrs Inhibition of TPA-induced Epstein-Barr virus early antigen activation in Raji cells after 48 hrs	[PMID: 17503850]
SK-BR-3	GI₅₀	22.9 μM Compound: 1, ATRA	Growth inhibition of human SKBR3 cells by MTT assay Growth inhibition of human SKBR3 cells by MTT assay	[PMID: 25634130]
T47D	IC₅₀	0.006 μM Compound: 1	Growth inhibition of human T47D cells after 6 days by MTT assay Growth inhibition of human T47D cells after 6 days by MTT assay	[PMID: 15615521]
T47D	GI₅₀	0.82 μM Compound: ATRA, Tretinoin	Cytotoxicity against human T47D cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human T47D cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 25701251]

体外研究
(In Vitro)

维甲酸（全反式维甲酸，ATRA）是维生素 A 的一种高效衍生物，几乎所有必需的生理过程和功能都需要维甲酸，因为它参与超过 530 种不同基因的转录调节。视黄酸通过作为核视黄酸受体 (RARα-γ) 的激活配体发挥其作用，核视黄酸受体与视黄酸 X 受体 (RXRα-γ) 形成异二聚体^[1]。
视黄酸 (RA) 以低亲和力与 PPARα 和 PPARγ 结合，K_d 值为 100-200 nM。相比之下，视黄酸与 PPARβ/δ 结合的 K_d 为 17 nM，显示出高亲和力和同种型选择性^[2]。
未分化的 P19 细胞表达视黄酸 (RA) 受体 RARα、RARβ、RARγ 和 PPARβ/δ，以及视黄酸结合蛋白 CRABP-II 和 FABP5。通过用视黄酸处理细胞诱导分化导致 CRABP-II 的瞬时上调和 FABP5 的下调，这在各自的蛋白质和 mRNA 水平上观察到。
在最初的下降之后，与未分化的 P19 细胞相比，成熟神经元中 FABP5 蛋白和 mRNA 的水平增加了 2-2.5 倍。诱导分化不会显着影响 RARα 或 PPARβ/δ 的水平。到第 4 天，RARγ mRNA 的水平降低了约 5 倍，并且在成熟神经元中保持较低水平^[3]。
视黄酸 (RA) 是一种来源于视黄醇（维生素 A）的形态发生素，在细胞生长、分化和器官发生中起重要作用。视黄酸与视黄酸受体 (RAR) 和视黄酸 X 受体 (RXR) 相互作用，进而调控靶基因的表达^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

300.44

Formula

C₂₀H₂₈O₂

CAS 号

302-79-4

性状

固体

颜色

Light yellow to yellow

中文名称

视黄酸；维生素A酸；维甲酸

结构分类

Ketones, Aldehydes, Acids

初始来源

微生物

内源性代谢物

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

细胞实验：

DMSO 中的溶解度 : 50 mg/mL (166.42 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.3285 mL	16.6423 mL	33.2845 mL
5 mM	0.6657 mL	3.3285 mL	6.6569 mL
10 mM	0.3328 mL	1.6642 mL	3.3285 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (8.32 mM); 悬浊液; 超声加热助溶

此方案可获得 2.5 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.32 mM); 悬浊液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.32 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。
方案四

请依序添加每种溶剂： 5% DMSO 40% PEG300 5% Tween-80 50% Saline
Solubility: 2.5 mg/mL (8.32 mM); 悬浊液; 超声助溶
方案五

请依序添加每种溶剂： 5% DMSO 95% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (8.32 mM); 悬浊液; 超声助溶

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： 50% PEG300 50% PBS
Solubility: 5 mg/mL (16.64 mM); 悬浊液; Need ultrasonic and warming and heat to 40°C

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.81%

选择批次：

Data Sheet (652 KB) SDS (701 KB)

COA (281 KB) 元素分析报告 (204 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Wu L, et al. Retinoid X Receptor Agonists Upregulate Genes Responsible for the Biosynthesis of All-Trans-Retinoic Acid in Human Epidermis. PLoS One. 2016 Apr 14;11(4):e0153556. [Content Brief]

[2]. Shaw N, et al. Retinoic acid is a high affinity selective ligand for the peroxisome proliferator-activated receptor beta/delta. J Biol Chem. 2003 Oct 24;278(43):41589-92. [Content Brief]

[3]. Yu S, et al. Retinoic acid induces neurogenesis by activating both retinoic acid receptors (RARs) and peroxisomeproliferator-activated receptor β/δ (PPARβ/δ). J Biol Chem. 2012 Dec 7;287(50):42195-205. [Content Brief]

[4]. Kam RK, et al. Retinoic acid synthesis and functions in early embryonic development. Cell Biosci. 2012 Mar 22;2(1):11. [Content Brief]

[5]. Apfel C, et al. A retinoic acid receptor alpha antagonist selectively counteracts retinoic acid effects. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7129-33. [Content Brief]

[6]. Xiu Jun Wang, et al. Identification of retinoic acid as an inhibitor of transcription factor Nrf2 through activation of retinoic acid receptor alpha. Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19589-94. [Content Brief]

Cell Assay ^[3]	P19 cell are induced to undergo neuronal differentiation according to established procedures. Briefly, cells are cultured on 1% agarose-coated 10 cm dishes at 3×10 ⁵ cells/mL in α-minimal essential medium supplemented with 10% FBS. Differentiation is induced by addition of Retinoic acid (1 μM) and medium containing Retinoic acid replaced 2 days later. On day 4, cell aggregates are collected by centrifugation, separated to single cells by trypsin/EDTA treatment, replated onto poly-L-lysine-coated plates, and cultured in α-minimal essential medium supplemented with 10% FBS. On day 6, medium is replaced with neurobasal medium containing B27 supplement and 2 mM GlutaMAX. Medium is replaced every 2 days for an additional week^[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Wu L, et al. Retinoid X Receptor Agonists Upregulate Genes Responsible for the Biosynthesis of All-Trans-Retinoic Acid in Human Epidermis. PLoS One. 2016 Apr 14;11(4):e0153556. [Content Brief] [2]. Shaw N, et al. Retinoic acid is a high affinity selective ligand for the peroxisome proliferator-activated receptor beta/delta. J Biol Chem. 2003 Oct 24;278(43):41589-92. [Content Brief] [3]. Yu S, et al. Retinoic acid induces neurogenesis by activating both retinoic acid receptors (RARs) and peroxisomeproliferator-activated receptor β/δ (PPARβ/δ). J Biol Chem. 2012 Dec 7;287(50):42195-205. [Content Brief] [4]. Kam RK, et al. Retinoic acid synthesis and functions in early embryonic development. Cell Biosci. 2012 Mar 22;2(1):11. [Content Brief] [5]. Apfel C, et al. A retinoic acid receptor alpha antagonist selectively counteracts retinoic acid effects. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7129-33. [Content Brief] [6]. Xiu Jun Wang, et al. Identification of retinoic acid as an inhibitor of transcription factor Nrf2 through activation of retinoic acid receptor alpha. Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19589-94. [Content Brief]

Cell Assay
^[3]

P19 cell are induced to undergo neuronal differentiation according to established procedures. Briefly, cells are cultured on 1% agarose-coated 10 cm dishes at 3×10 ⁵ cells/mL in α-minimal essential medium supplemented with 10% FBS. Differentiation is induced by addition of Retinoic acid (1 μM) and medium containing Retinoic acid replaced 2 days later. On day 4, cell aggregates are collected by centrifugation, separated to single cells by trypsin/EDTA treatment, replated onto poly-L-lysine-coated plates, and cultured in α-minimal essential medium supplemented with 10% FBS. On day 6, medium is replaced with neurobasal medium containing B27 supplement and 2 mM GlutaMAX. Medium is replaced every 2 days for an additional week^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Wu L, et al. Retinoid X Receptor Agonists Upregulate Genes Responsible for the Biosynthesis of All-Trans-Retinoic Acid in Human Epidermis. PLoS One. 2016 Apr 14;11(4):e0153556. [Content Brief]

[2]. Shaw N, et al. Retinoic acid is a high affinity selective ligand for the peroxisome proliferator-activated receptor beta/delta. J Biol Chem. 2003 Oct 24;278(43):41589-92. [Content Brief]

[3]. Yu S, et al. Retinoic acid induces neurogenesis by activating both retinoic acid receptors (RARs) and peroxisomeproliferator-activated receptor β/δ (PPARβ/δ). J Biol Chem. 2012 Dec 7;287(50):42195-205. [Content Brief]

[4]. Kam RK, et al. Retinoic acid synthesis and functions in early embryonic development. Cell Biosci. 2012 Mar 22;2(1):11. [Content Brief]

[5]. Apfel C, et al. A retinoic acid receptor alpha antagonist selectively counteracts retinoic acid effects. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7129-33. [Content Brief]

[6]. Xiu Jun Wang, et al. Identification of retinoic acid as an inhibitor of transcription factor Nrf2 through activation of retinoic acid receptor alpha. Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19589-94. [Content Brief]

Retinoic acid 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.3285 mL	16.6423 mL	33.2845 mL	83.2113 mL
	5 mM	0.6657 mL	3.3285 mL	6.6569 mL	16.6423 mL
	10 mM	0.3328 mL	1.6642 mL	3.3285 mL	8.3211 mL
	15 mM	0.2219 mL	1.1095 mL	2.2190 mL	5.5474 mL
	20 mM	0.1664 mL	0.8321 mL	1.6642 mL	4.1606 mL
	25 mM	0.1331 mL	0.6657 mL	1.3314 mL	3.3285 mL
	30 mM	0.1109 mL	0.5547 mL	1.1095 mL	2.7737 mL
	40 mM	0.0832 mL	0.4161 mL	0.8321 mL	2.0803 mL
	50 mM	0.0666 mL	0.3328 mL	0.6657 mL	1.6642 mL
	60 mM	0.0555 mL	0.2774 mL	0.5547 mL	1.3869 mL
	80 mM	0.0416 mL	0.2080 mL	0.4161 mL	1.0401 mL
	100 mM	0.0333 mL	0.1664 mL	0.3328 mL	0.8321 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Retinoic acid302-79-4Vitamin A acid all-trans-Retinoic acid ATRA视黄酸维生素A酸维甲酸OrganoidRAR/RXRPPAREndogenous MetaboliteAutophagyRetinoic acid receptorsRetinoid X receptorsPeroxisome proliferator-activated receptorsInhibitorinhibitorinhibit

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Retinoic acid (Synonyms: 维生素A酸; Vitamin A acid; all-trans-Retinoic acid; ATRA)

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Other Forms of Retinoic acid:

MCE 顾客使用本产品发表的 62 篇科研文献

Retinoic acid 相关产品

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参考文献

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Retinoic acid (Synonyms: 维生素A酸; Vitamin A acid; all-trans-Retinoic acid; ATRA)

Customer Review

Other Forms of Retinoic acid:

Retinoic acid 相关抗体

MCE 顾客使用本产品发表的 62 篇科研文献

Retinoic acid 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 RAR/RXR 亚型特异性产品:

查看 PPAR 亚型特异性产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

Retinoic acid 相关抗体:

摩尔计算器

稀释计算器

Retinoic acid 相关分类

完整储备液配制表

Keywords:

您最近查看的产品:

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