2. Apoptosis Epigenetics Cell Cycle/DNA Damage Cytoskeleton Metabolic Enzyme/Protease Immunology/Inflammation NF-κB
  3. Apoptosis HDAC Microtubule/Tubulin Reactive Oxygen Species
  4. Tubulin/HDAC-IN-4

Tubulin/HDAC-IN-4 (compound 9n) 是一种微管 (Tubulin) 和 HDAC 双重抑制剂，对 HDAC1、HDAC2、HDAC6、HDAC7 的 IC50 值分别为 0.73、0.43、0.62、2.34 µM。Tubulin/HDAC-IN-4 通过靶向秋水仙碱结合位点抑制微管蛋白聚合。Tubulin/HDAC-IN-4 诱导细胞凋亡 (apoptosis) 和细胞周期停滞在 G2/M 期。Tubulin/HDAC-IN-4 诱导细胞内 ROS 水平显着升高。Tubulin/HDAC-IN-4 显示出抗血管生成活性和抗癌活性。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Tubulin/HDAC-IN-4 Chemical Structure

Tubulin/HDAC-IN-4 Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

Tubulin/HDAC-IN-4 相关抗体

Top Publications Citing Use of Products

查看 HDAC 亚型特异性产品:

查看所有亚型
HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Tubulin/HDAC-IN-4 (compound 9n) is a dual Tubulin and HDAC inhibitor with IC50 values of 0.73, 0.43, 0.62, 2.34 µM for HDAC1, HDAC2, HDAC6, HDAC7, respectively. Tubulin/HDAC-IN-4 inhibits the tubulin polymerization by targeting the colchicine binding site. Tubulin/HDAC-IN-4 induces apoptosis and cell cycle arrest at G2/M phase. Tubulin/HDAC-IN-4 induces a significant elevation of intracellular ROS levels. Tubulin/HDAC-IN-4 shows anti-angiogenesis activity and anticancer activity[1].

IC50 & Target[1]

HDAC1

0.73 μM (IC50)

HDAC2

0.43 μM (IC50)

HDAC6

0.62 μM (IC50)

HDAC7

2.34 μM (IC50)

体外研究
(In Vitro)

Tubulin/HDAC-IN-4 (compound 9n) (0-10 µM; 72 h) 对 MDA-MB-231、A549 表现出细胞毒性，IC50s 为 0.34、0.29、0.016、0.15、0.16 µM 分别为 PC-3、U251、MCF-7 细胞[1]。
Tubulin/HDAC-IN-4（2.5、5、10、20、40 nM；24 小时）以剂量依赖性方式抑制 PC-3 细胞集落形成[1]。
Tubulin/HDAC-IN-4 (0.2、1、5、25 µM) 抑制微管蛋白聚合，IC50值为 4.82 µM[1]。
Tubulin/HDAC-IN-4（0.08、0.16、0.32；24 h）可增加 PC-3 细胞中 Ac-α-tubulin 和 Ac-Histone H3 的表达[1]。
Tubulin/HDAC-IN-4（0.08、0.16、0.32 µM；24 小时）诱导细胞凋亡和 G2/M 期细胞周期停滞[1]。
Tubulin/HDAC-IN-4（0.08、0.16、0.32 µM；24 小时）可诱导细胞内 ROS 水平显着升高[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Tubulin/HDAC-IN-4 相关抗体:

Cell Cytotoxicity Assay[1]

Cell Line: MDA-MB-231, A549, PC-3, U251, MCF-7 cells
Concentration: 0-10 µM
Incubation Time: 72 h
Result: Showed cytotoxicity with IC50s of 0.34, 0.29, 0.016, 0.15, 0.16 µM for MDA-MB-231, A549, PC-3, U251, MCF-7 cells, respectively.

Western Blot Analysis[1]

Cell Line: PC-3 cells
Concentration: 0.08, 0.16, 0.32 µM
Incubation Time: 24 h
Result: Increased in both the expression of HDAC6 substrate Ac-α-tubulin and HDAC1/2/3 substrate Ac-Histone H3 in a dose-dependent manner.

Cell Cycle Analysis[1]

Cell Line: PC-3 cells
Concentration: 0.08, 0.16, 0.32 µM
Incubation Time: 24 h
Result: Dose-dependently arrested PC-3 cells at G2/M phase, decreased in expression level of p-Cdc25cSer216, p-Cdc2Thr216 and p-CdcTyr15, increased the expression of Cyclin B1.

Apoptosis Analysis[1]

Cell Line: PC-3 cells
Concentration: 0.08, 0.16, 0.32 µM
Incubation Time: 24 h
Result: Induced apoptosis and increased the expression of cleaved PARP and cleaved Caspase 3, decreased the expression of Bim and Bcl-2.
体内研究
(In Vivo)

Tubulin/HDAC-IN-4 (10, 20 mg/kg; i.v.; every two days for 21 days) 显示出抗癌活性[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5-week-old male BALB/c nude mouse (PC-3 cells)[1]
Dosage: 10, 20 mg/kg
Administration: I.v.; every two days for 21 days
Result: Inhibited the growth of tumor with the tumor growth inhibition (TGI) reached 90.07% at 20 mg/kg.
分子量

438.47

Formula

C24H26N2O6
Unlabeled Cas

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Tubulin/HDAC-IN-4 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tubulin/HDAC-IN-4ApoptosisHDACMicrotubule/TubulinReactive Oxygen SpeciesHistone deacetylasesapoptosisROS levelsG2/MPC-3 cellsInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
Tubulin/HDAC-IN-4
目录号：
HY-162319
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z