Frizzled-9 蛋白, Mouse (HEK293, mFc)

Frizzled-9, a WNT2 receptor, crucially activates the beta-catenin pathway, involving disheveled protein activation, GSK-3 kinase inhibition, nuclear beta-catenin accumulation, and Wnt target gene activation. Beyond canonical signaling, it negatively regulates acetylcholine receptor clustering in neuromuscular junction assembly and influences neural progenitor cell viability by regulating the cell cycle. In hippocampal development, Frizzled-9 regulates neuroblast proliferation and apoptosis. Additionally, it plays a pivotal role in bone dynamics, modulating bone formation and regeneration through non-canonical Wnt signaling pathways. Frizzled-9 Protein, Mouse (HEK293, mFc) is the recombinant mouse-derived Frizzled-9 protein, expressed by HEK293, with C-mFc labeled tag. The total length of Frizzled-9 Protein, Mouse (HEK293, mFc) is 163 a.a., with molecular weight of 52-61 kDa.

Frizzled-9 是 WNT2 的受体，是 β-连环蛋白经典信号通路中的关键角色，协调蓬乱蛋白的激活、GSK-3 激酶的抑制、β-连环蛋白的核积累以及随后的 Wnt 激活目标基因。除了在经典 Wnt 信号传导中的作用之外，Frizzled-9 还表现出多方面的功能。它通过 β-连环蛋白途径负向调节乙酰胆碱受体 (AChR) 的聚集，从而有助于神经肌肉接头 (NMJ) 的组装。在神经祖细胞 (NPC) 中，Frizzled-9 通过负向调节细胞周期停滞来影响细胞活力，从而抑制细胞凋亡过程。该受体参与海马发育包括调节神经母细胞增殖和凋亡细胞死亡。此外，Frizzled-9 在骨动力学中发挥着关键作用，通过 ISG15 介导的非经典 Wnt 信号传导调节骨形成，并通过非经典 Wnt 信号传导途径对骨再生产生积极影响。

Measured by its ability to bind biotinylated mouse Wnt-3a in a funtional ELISA with an estimated Kd 2.263 nM.

• 
  Immobilized Frizzled-9 at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse Wnt3a protein. The Kd is 2.263 nM.

Mouse

HEK293

C-mFc

Q9R216 (L24-D186)

14371  [NCBI]

LEIGRFDPERGRGPAPCQAMEIPMCRGIGYNLTRMPNLLGHTSQGEAAAQLAEFSPLVQYGCHSHLRFFLCSLYAPMCTDQVSTPIPACRPMCEQARLRCAPIMEQFNFGWPDSLDCARLPTRNDPHALCMEAPENATAGPTEPHKGLGMLPVAPRPARPPGD

52-61 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 500 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.