Trigger factor 蛋白, E.coli (P.pastoris, His)

Trigger protein is essential for protein export, acting as chaperone and peptidyl prolyl cis-trans isomerase. In its chaperone role, it keeps nascent polypeptides in an open conformation, facilitating correct protein folding. Trigger factor Protein, E.coli (P.pastoris, His) is the recombinant E. coli-derived Trigger factor protein, expressed by P. pastoris , with N-His labeled tag.

触发因子蛋白是蛋白质输出的组成部分，具有双重作用：作为伴侣，帮助新合成的蛋白质正确折叠；以及作为肽基-脯氨酰顺反异构酶。在其伴侣能力中，触发因子在维持新生多肽处于开放构象、促进复杂的蛋白质折叠过程中发挥着至关重要的作用。同时，其肽基-脯氨酰顺反异构酶活性有助于脯氨酰肽键的异构化，进一步完善新兴蛋白质的构象景观。这种多方面的参与强调了触发因子在细胞蛋白质稳态和输出机制的复杂协调中的重要性。

E.coli

P. pastoris

N-His

B7UJQ9 (M1-A432)

58463319  [NCBI]

MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFRKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGEDFTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFPEEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMERELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPRELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNKELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA

Approximately 60 kDa.The reducing (R) protein migrat es as 60 kDa in SDS-PAGE may be due to relative charge.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in Tris-based buffer, 50% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.