Glypican-1/GPC1 蛋白, Human (HEK293, His)

Glypican-1 (GPC1) is a heparan sulfate-containing cell surface proteoglycan. Glypican-1/GPC1 Protein, Human (HEK293, His) is the recombinant human-derived Glypican-1/GPC1 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of Glypican-1/GPC1 Protein, Human (HEK293, His) is 506 a.a., with molecular weight of 65-150 kDa.

Glypican-1 (GPC1) 蛋白是一种细胞表面蛋白聚糖，其特征是与硫酸乙酰肝素相关。它通过硫酸乙酰肝素侧链结合 alpha-4 (V) 胶原蛋白，并在雪旺细胞髓鞘形成中发挥作用。此外，GPC1 可以作为朊病毒蛋白 PRPN(C) 转化为 PRNP(Sc) 的催化剂，通过与两种形式的 PRPN 结合，将它们靶向脂筏，并促进它们的相互作用。此外，GPC1 对于正确的骨骼肌分化至关重要，因为它将 FGF2 隔离在脂筏中，防止其与受体 (FGFR) 结合并抑制 FGF 介导的信号传导。

Human

HEK293

N-6*His

P35052 (D24-T529)

2817  [NCBI]

DPASKSRSCGEVRQIYGAKGFSLSDVPQAEISGEHLRICPQGYTCCTSEMEENLANRSHAELETALRDSSRVLQAMLATQLRSFDDHFQHLLNDSERTLQATFPGAFGELYTQNARAFRDLYSELRLYYRGANLHLEETLAEFWARLLERLFKQLHPQLLLPDDYLDCLGKQAEALRPFGEAPRELRLRATRAFVAARSFVQGLGVASDVVRKVAQVPLGPECSRAVMKLVYCAHCLGVPGARPCPDYCRNVLKGCLANQADLDAEWRNLLDSMVLITDKFWGTSGVESVIGSVHTWLAEAINALQDNRDTLTAKVIQGCGNPKVNPQGPGPEEKRRRGKLAPRERPPSGTLEKLVSEAKAQLRDVQDFWISLPGTLCSEKMALSTASDDRCWNGMARGRYLPEVMGDGLANQINNPEVEVDITKPDMTIRQQIMQLKIMTNRLRSAYNGNDVDFQDASDDGSGSGSGDGCLDDLCSRKVSRKSSSSRTPLTHALPGLSEQEGQKT

65-150 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.