Hsp40 抗体

Hsp40 Antibody is a non-conjugated and Mouse origined monoclonal antibody about 38 kDa, targeting to Hsp40. It can be used for WB assays with tag free, in the background of Human.

Hsp40：该基因编码 DnaJ 或 Hsp40（热休克蛋白 40 kD）蛋白质家族的成员。DNAJ 家族成员的特点是高度保守的氨基酸延伸称为“J 结构域”，作为参与广泛细胞事件（如蛋白质折叠和寡聚蛋白质复合物组装）的两大类分子伴侣之一发挥作用。编码的蛋白质是一种分子伴侣，可刺激 Hsp70 热休克蛋白的 ATP 酶活性，以促进蛋白质折叠并防止错误折叠的蛋白质聚集。可变剪接导致多个转录本变体。[由 RefSeq 提供，2015 年 9 月]

Free

3337

Predicted band size: 38 kDa;Observed band size: 38 kDa

affinity purified

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

P25685

WB: 1:500-1:1,000

• 
  Western blot analysis of extracts from Jurkat(lane 2(20μg) or lane 3(40μg) ) and Hela(lane 4(20μg) or lane 5(40μg) ) using Hsp40(HY-P80710) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80710, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Hsp40.The exact sequence is proprietary to MCE.