1. Academic Validation
  2. Development of monoclonal antibodies against pirimiphos-methyl and their application to IC-ELISA

Development of monoclonal antibodies against pirimiphos-methyl and their application to IC-ELISA

  • J Agric Food Chem. 2006 Jun 28;54(13):4551-6. doi: 10.1021/jf0606196.
Zheng-You Yang 1 Anna Yu Kolosova Won-Bo Shim Duck-Hwa Chung
Affiliations

Affiliation

  • 1 Division of Applied Life Science, Graduate School of Gyeongsan National University, Chinju, Gyeongnam 660-701, South Korea.
Abstract

To detect the organophosphorus (OP) pesticide pirimiphos-methyl in grain samples, a monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed and optimized. By the active esters method, pirimiphos-methyl Hapten A was conjugated to keyhole limpet hemocyanin to be used as the immunogen for the production of monoclonal Antibodies, and pirimiphos-methyl Hapten B was conjugated to ovalbumin to be used as coating antigen. By using the monoclonal antibody and the coating antigen, an IC-ELISA has been developed. Under the established optimized conditions, the IC-ELISA showed an IC50 of 4.2 ng/mL with a detection limit of 0.07 ng/mL. The IC-ELISA showed negligible cross-reactivity with other OP pesticides except with pirimiphos-ethyl. Recoveries of pirimiphos-methyl from spiked grain samples ranged from 83 to 96%.

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