The influence of octyl β-D-glucopyranoside on cell lysis induced by ultrasonic cavitation

Octyl β-D-glucopyranoside (OGP) has been reported to completely inhibit cavitation-induced Cell Lysis in vitro, possibly by quenching critical free-radical effects. In this study, the influence of OGP on Cell Lysis in a 60 rpm rotating-tube exposure apparatus was assessed. HL-60 Cell Lysis was estimated with a Coulter Multisizer counter. Cavitation activity from the 2.3 MHz, 30 s duration exposures were monitored at the 1.15 MHz subharmonic. Cavitation nucleation was accomplished by addition of an ultrasound contrast agent, or by using freshly dissolved culture media. For both nucleation methods, exposures were conducted for 0-0.7 MPa peak rarefactional pressure-amplitudes with and without 5 mM OGP, and for 0.5 MPa with 0-5 mM OGP. The addition of OGP to the cell suspension medium generally had little influence on cavitation-induced Cell Lysis. Exposures with no rotation had reduced subharmonic and lysis for added contrast agent, but essentially no cavitation for the fresh medium. Since the decreases or increases in Cell Lysis found for added OGP generally were accounted for by concomitant decreases or increases in cavitation activity, the changes in Cell Lysis could be explained by variation of the mechanical effects of cavitation without invoking a critical role for free-radical effects.