1. Academic Validation
  2. Pertussis toxin sensitivity of drug-induced potentials on the rat superior cervical ganglion

Pertussis toxin sensitivity of drug-induced potentials on the rat superior cervical ganglion

  • Eur J Pharmacol. 1989 Apr 25;163(2-3):245-52. doi: 10.1016/0014-2999(89)90193-3.
N R Newberry 1 M J Gilbert
Affiliations

Affiliation

  • 1 Merck Sharp & Dohme Research Laboratories, Neuroscience Research Centre, Harlow, Essex, U.K.
Abstract

We have investigated the action of pertussis toxin on a range of receptor-mediated responses of the rat superior cervical ganglion in vitro. The ganglia were treated with pertussis toxin for 24 h at 37 degrees C using an in vitro method. Appropriate controls were also carried out. Pertussis toxin (1 microgram/ml) reduced ganglionic hyperpolarisations mediated by adenosine, alpha 2, 5-HT1A, M2 and GABAB receptors. The GABAB-mediated hyperpolarisation of this preparation, evoked by baclofen and GABA in a bicuculline-resistant manner, has not previously been reported. Pertussis toxin did not reduce ganglionic depolarisations evoked by potassium chloride and 5-HT3, GABAA and nicotinic receptors. Depolarisations to muscarine and noradrenaline, probably mediated by M1 and beta-receptors, also appeared to be resistant to pertussis toxin. The similar sensitivity of the various ganglionic hyperpolarisations to pertussis toxin indicates that they may all be mediated by similar G-proteins.

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