2. Academic Validation
  3. Biosynthesis of advanced biofuel farnesyl acetate using engineered Escherichia coli

Biosynthesis of advanced biofuel farnesyl acetate using engineered Escherichia coli

  • Bioresour Technol. 2018 Dec;269:577-580. doi: 10.1016/j.biortech.2018.08.112.
Daoyi Guo 1,  Sijia Kong 1,  Lihua Zhang 1,  Hong Pan 2,  Chao Wang 3,  Zhijie Liu 4
Affiliations

Affiliations

  • 1 Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Gannan Normal University, Ganzhou 341000, China.
  • 2 Key Laboratory of Organo-Pharmaceutical Chemistry, Jiangxi Province, Gannan Normal University, Ganzhou 341000, China. Electronic address: panhong_813@163.com.
  • 3 Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, Hubei Collaborative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China.
  • 4 Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, Hubei Collaborative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China. Electronic address: zjliu@hbut.edu.cn.
PMID: 30181019 DOI: 10.1016/j.biortech.2018.08.112
Abstract

Diminishing petroleum reserves and the rapid accumulation of greenhouse gases lead to increasing interest in microbial biofuels. In this study, a heterologous farnesyl acetate biosynthesis pathway was constructed in Escherichia coli for the first time. Firstly, the AtoB, ERG13, tHMG1, ERG12, ERG8, MVD1, Idi, IspA and PgpB were expressed to accumulate farnesol in the E. coli cells. Then the alcohol acetyltransferase (ATF1) was heterologous overexpressed for the subsequent esterification farnesol to farnesyl acetate. The engineered strain DG 106 accumulated 128 ± 10.5 mg/L of farnesyl acetate. Finally, the isopentenyl-diphosphate isomerase was further overexpressed, and the recombinant strain DG107 produced 201 ± 11.7 mg/L of farnesyl acetate. This study shows the novel method for the biosynthesis of the advanced biofuel farnesyl acetate directly from glucose and highlight the enormous designing strategies for metabolic engineering of bioproducts.

Keywords

Alcohol acetyltransferase; Escherichia coli; Farnesol; Farnesyl acetate.

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