2. Academic Validation
  3. Screening of PI3K-Akt-targeting Drugs for Silkworm against Bombyx mori Nucleopolyhedrovirus

Screening of PI3K-Akt-targeting Drugs for Silkworm against Bombyx mori Nucleopolyhedrovirus

  • Molecules. 2019 Apr 1;24(7):1260. doi: 10.3390/molecules24071260.
Bingbing Wang 1 2 3,  Liang Jiang 4 5 6,  Huizhen Guo 7 8 9,  Qiang Sun 10 11 12,  Yumei Wang 13 14 15,  Enyu Xie 16 17 18,  Qingyou Xia 19 20 21
Affiliations

Affiliations

  • 1 Biological Science Research Center, Southwest University, Chongqing 400716, China. wb7532@email.swu.edu.cn.
  • 2 Chongqing Key Laboratory of Sericultural Science, Chongqing 400716, China. wb7532@email.swu.edu.cn.
  • 3 Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China. wb7532@email.swu.edu.cn.
  • 4 Biological Science Research Center, Southwest University, Chongqing 400716, China. jiangliang@swu.edu.cn.
  • 5 Chongqing Key Laboratory of Sericultural Science, Chongqing 400716, China. jiangliang@swu.edu.cn.
  • 6 Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China. jiangliang@swu.edu.cn.
  • 7 Biological Science Research Center, Southwest University, Chongqing 400716, China. guohuizhen.111@gmail.com.
  • 8 Chongqing Key Laboratory of Sericultural Science, Chongqing 400716, China. guohuizhen.111@gmail.com.
  • 9 Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China. guohuizhen.111@gmail.com.
  • 10 Biological Science Research Center, Southwest University, Chongqing 400716, China. sq35@email.swu.edu.cn.
  • 11 Chongqing Key Laboratory of Sericultural Science, Chongqing 400716, China. sq35@email.swu.edu.cn.
  • 12 Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China. sq35@email.swu.edu.cn.
  • 13 Biological Science Research Center, Southwest University, Chongqing 400716, China. wdwym7@email.swu.edu.cn.
  • 14 Chongqing Key Laboratory of Sericultural Science, Chongqing 400716, China. wdwym7@email.swu.edu.cn.
  • 15 Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China. wdwym7@email.swu.edu.cn.
  • 16 Biological Science Research Center, Southwest University, Chongqing 400716, China. xieenyu@outlook.com.
  • 17 Chongqing Key Laboratory of Sericultural Science, Chongqing 400716, China. xieenyu@outlook.com.
  • 18 Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China. xieenyu@outlook.com.
  • 19 Biological Science Research Center, Southwest University, Chongqing 400716, China. xiaqy@swu.edu.cn.
  • 20 Chongqing Key Laboratory of Sericultural Science, Chongqing 400716, China. xiaqy@swu.edu.cn.
  • 21 Chongqing Engineering and Technology Research Center for Novel Silk Materials, Chongqing 400716, China. xiaqy@swu.edu.cn.
PMID: 30939726 DOI: 10.3390/molecules24071260
Abstract

Bombyx mori nucleopolyhedrovirus (BmNPV) is the most prevalent threat to silkworms. Hence, there is a need for Antiviral agents in sericulture. The PI3K-Akt pathway is essential for the efficient replication of the baculovirus. In an attempt to screen Antiviral drugs against BmNPV, we summarized the commercial compounds targeting PI3K-Akt and selected the following seven oral drugs for further analyses: afuresertib, AZD8835, AMG319, HS173, AS605240, GDC0941, and BEZ235. Cell viability assay revealed that the cytotoxicity of these drugs at 10 µM concentration was not strong. Viral fluorescence observation and qPCR analysis showed that these candidate drugs significantly inhibited BmNPV in BmE cells. Only AMG319 and AZD8835 inhibited viral proliferation in silkworm larvae. The mortality of AZD8835-treated silkworms was lower than that of the control silkworms. Western blotting showed that AMG319 and AZD8835 decreased p-Akt expression after BmNPV Infection. These results suggest that AZD8835 has application potential in sericulture.

Keywords

AZD8835; BmNPV; PI3K-Akt; antivirus; silkworm.

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