2. Academic Validation
  3. An optimized cocktail of chitinolytic enzymes to produce N,N'-diacetylchitobiose and N-acetyl-d-glucosamine from defatted krill by-products

An optimized cocktail of chitinolytic enzymes to produce N,N'-diacetylchitobiose and N-acetyl-d-glucosamine from defatted krill by-products

  • Int J Biol Macromol. 2019 Jul 15;133:1029-1034. doi: 10.1016/j.ijbiomac.2019.04.114.
Fangmeng Chu 1,  Di Wang 1,  Tian Liu 2,  Hongyu Han 1,  Yike Yu 3,  Qing Yang 4
Affiliations

Affiliations

  • 1 State Key Laboratory of Fine Chemical Engineering and School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
  • 2 State Key Laboratory of Fine Chemical Engineering and School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China. Electronic address: tianliu@dlut.dut.cn.
  • 3 Liaoyu Group Co., Ltd., Dalian 116113, China.
  • 4 State Key Laboratory of Fine Chemical Engineering and School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China; Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: qingyang@dlut.edu.cn.
PMID: 31004644 DOI: 10.1016/j.ijbiomac.2019.04.114
Abstract

Defatted krill powder (DKP), the byproduct of krill oil industry, is a resource of biological macromolecules. Here, one Bacterial protease, three Bacterial chitinases and one insect N-acetyl-d-hexosaminidase were integratively used to produce peptide, N,N'-diacetylchitobiose [(GlcNAc)2] and N-acetyl-d-glucosamine (GlcNAc) from DKP. First, alkaline Protease was found to outperform neutral Protease in deproteinizing DKP and the resultant krill Peptides were rich in essential Amino acids (41.4%). Second, the mutant of chitinase A from Serratia marcescens [SmChiA-F232W/F396W (SmChiA-M)] was found to be 32% faster than wild-type SmChiA in hydrolyzing the deproteinized DKP (DDKP) and showed significant synergy with chitinase B from S. marcescens (SmChiB) and chitinase C from S. marcescens (SmChiC). Then two SmChiA-M-based Enzyme combinations [SmChiA-M + SmChiB + SmChiC and SmChiA-M + SmChiB + SmChiC + OfHex1 (an insect N-acetyl-d-hexosaminidase from Ostrinia furnacalis)] were designed to produce (GlcNAc)2 and GlcNAc, respectively, from DDKP. A yield of 2.04 g/L (GlcNAc)2 or 2.71 g/L GlcNAc (each with 95% purity) could be obtained from 20 g/L DDKP in 24 h.

Keywords

Antarctic krill; Chitin; Chitinase; N-acetyl-d-glucosamine; Protease.

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