1. Academic Validation
  2. Beneficial effect of 2'-acetylacteoside on ovariectomized mice via modulating the function of bone resorption

Beneficial effect of 2'-acetylacteoside on ovariectomized mice via modulating the function of bone resorption

  • Biomed Pharmacother. 2020 Nov;131:110747. doi: 10.1016/j.biopha.2020.110747.
Yanting Li 1 Nan Li 1 Xiaojun Zhao 1 Bo Zhang 1 Lingling Yang 1 Jingjing Liu 1 Hunter Snooks 2 Changling Hu 2 Xueqin Ma 3
Affiliations

Affiliations

  • 1 Department of Pharmaceutical Analysis, School of Pharmacy, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, 1160 Shenli Street, Yinchuan, 750004, China.
  • 2 Laboratory for Functional Foods and Human Health, Center for Excellence in Post Harvest Technologies, North Caroline A & T State University, North Carolina Research Campus, 500 Laureate Way, Kannapolis, NC, 28081, USA.
  • 3 Department of Pharmaceutical Analysis, School of Pharmacy, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, 1160 Shenli Street, Yinchuan, 750004, China. Electronic address: maxueqin217@126.com.
Abstract

2'-Acetylacteoside-(2'-AA), a bioactive constituent isolated from Cistanche deserticola, has been proven to possess a variety of important pharmacological effects, thus brought an increased amount of scientists' attention. As the extract of C. deserticola exhibited significant anti-osteoporotic bioactivity in our previous study, we proposed that 2'-AA maybe one of the responsibilities. As a result, 2'-AA (10, 20 and 40 mg/kg body weight/day) exhibited significant anti-osteoporotic effects on ovariectomized (OVX) mice after 12 weeks of oral administration, confirmed by the increased bone mineral density, enhanced bone strength and improved trabecular bone micro-architecture including bone mineral content, tissue mineral content, trabecular number, and trabecular separation of OVX mice. Moreover, the properties of bone resorption markers including Cathepsin K, TRAP and deoxypyridinoline were significantly suppressed, whereas the activities of bone formation index like ALP and BGP as well as the weights of the body, uterus, and vagina were seemingly not influenced by 2'-AA intervention. Mechanistically, the above therapeutic effect of 2'-AA on bone resorption of OVX mice operated maybe mainly through RANKL/RANK/TRAF6-mediated NF-κB/NFATc1 pathway, which was confirmed by the down-regulated expressions of RANK, TRAF6, IκB kinase β, NF-κB and NFATc1. Summarily, 2'-AA exhibited significant anti-osteoporotic activity and may be regarded as a promising anti-osteoporotic candidate for future clinical trial.

Keywords

2’-Acetylacteoside; Anti-osteoporotic; Ovariectomized mice; RANK; TRAF6.

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