2. Academic Validation
  3. Cell-based and cell-free biocatalysis for the production of D-glucaric acid

Cell-based and cell-free biocatalysis for the production of D-glucaric acid

  • Biotechnol Biofuels. 2020 Dec 10;13(1):203. doi: 10.1186/s13068-020-01847-0.
Lu-Zhou Chen 1,  Si-Ling Huang 2,  Jin Hou 3,  Xue-Ping Guo 2,  Feng-Shan Wang 1 4,  Ju-Zheng Sheng 5 6
Affiliations

Affiliations

  • 1 Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China.
  • 2 Bloomage BioTechnology Corp., Ltd., Jinan, 250010, China.
  • 3 The State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China.
  • 4 National Glycoengineering Research Center, Shandong University, Jinan, 250012, China.
  • 5 Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China. Shengjuzheng@sdu.edu.cn.
  • 6 National Glycoengineering Research Center, Shandong University, Jinan, 250012, China. Shengjuzheng@sdu.edu.cn.
PMID: 33303009 DOI: 10.1186/s13068-020-01847-0
Abstract

D-Glucaric acid (GA) is a value-added chemical produced from biomass, and has potential applications as a versatile platform chemical, food additive, metal sequestering agent, and therapeutic agent. Marketed GA is currently produced chemically, but increasing demand is driving the search for eco-friendlier and more efficient production approaches. Cell-based production of GA represents an alternative strategy for GA production. A series of synthetic pathways for GA have been ported into Escherichia coli, Saccharomyces cerevisiae and Pichia pastoris, respectively, and these engineered cells show the ability to synthesize GA de novo. Optimization of the GA metabolic pathways in host cells has leapt forward, and the titer and yield have increased rapidly. Meanwhile, cell-free multi-enzyme catalysis, in which the desired pathway is constructed in vitro from Enzymes and cofactors involved in GA biosynthesis, has also realized efficient GA bioconversion. This review presents an overview of studies of the development of cell-based GA production, followed by a brief discussion of potential applications of biosensors that respond to GA in these biosynthesis routes.

Keywords

Biosensor; Cell-free synthetic biology; D-Glucaric acid; Escherichia coli; Metabolic engineering; Yeast.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z