1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. aKMT 蛋白, Sulfolobus islandicus

aKMT 蛋白, Sulfolobus islandicus

目录号: HY-P701940
产品使用指南

赖氨酸甲基转移酶 (aKMT) 负责催化靶蛋白中赖氨酸残基的甲基化,使用 S-腺苷-L-甲硫氨酸 (SAM) 作为甲基供体。该酶表现出广泛的底物特异性,在体外表现出主要在穹窿染色质蛋白 Cren7 的“Lys-11”、“Lys-16”和“Lys-31”处以及几种重组硫化叶菌蛋白的甲基化能力。aKMT 蛋白, Sulfolobus islandicus 是重组的 aKMT 蛋白,由 E. coli 表达,不带标签。aKMT 蛋白, Sulfolobus islandicus 全长 160 个氨基酸。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Lysine methyltransferase (aKMT) is responsible for catalyzing the methylation of lysine residues in target proteins, using S-adenosyl-L-methionine (SAM) as a methyl donor. The enzyme exhibits broad substrate specificity, showing methylation primarily at "Lys-11", "Lys-16" and "Lys-31" of the vault chromatin protein Cren7 as well as several recombinant Sulfolobus proteins in vitro Ability. aKMT Protein, Sulfolobus islandicus is the recombinant aKMT protein, expressed by E. coli , with tag free. The total length of aKMT Protein, Sulfolobus islandicus is 160 a.a., .

研究背景

aKMT 蛋白充当赖氨酸甲基转移酶,催化靶蛋白中赖氨酸残基的甲基化。这种酶活性是使用 S-腺苷-L-甲硫氨酸 (SAM) 作为甲基供体来完成的。aKMT 显示出广泛的底物特异性,并且能够甲基化各种靶蛋白。特别是,已观察到在特定的赖氨酸残基(包括“Lys-11”、“Lys-16”和“Lys-31”)处甲基化了穹窿染色质蛋白 Cren7。此外,aKMT 表现出序列无关的赖氨酸甲基化,展示了其在体外修饰一系列靶蛋白方面的多功能性。

种属

Others

表达系统

E. coli

标签

Tag Free

蛋白编号

F0NBH8 (S2-K161)

基因 ID

8761611

蛋白结构
N-term
aKMT (S2-K161)
Accession # F0NBH8
C-term
同用名
Protein-lysine N-methyltransferase; Archaeal protein lysine methyltransferase; aKMT
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

aKMT Protein, Sulfolobus islandicus 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
aKMT Protein, Sulfolobus islandicus
目录号:
HY-P701940
需求量: