1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. Aldose-reductase 蛋白, Human (His)

Aldose-reductase 蛋白, Human (His)

目录号: HY-P701973
产品使用指南

醛糖还原酶蛋白将含羰基的化合物还原为醇,包括视黄醛。它可以解毒巴豆醛和 4-羟基壬烯醛等不饱和羰基化合物,但缺乏对葡萄糖的还原酶活性。Aldose-reductase 蛋白, Human (His) 是重组的 醛糖还原酶蛋白,由 E. coli 表达,带有 N-6*His 标签。Aldose-reductase 蛋白, Human (His) 全长 316 个氨基酸。

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描述

The Aldose-reductase protein reduces carbonyl-containing compounds to alcohols, including retinals. It detoxifies unsaturated carbonyls like crotonaldehyde and 4-hydroxynonenal, but lacks reductase activity towards glucose. Aldose-reductase Protein, Human (His) is the recombinant human-derived Aldose-reductase protein, expressed by E. coli , with N-6*His labeled tag. The total length of Aldose-reductase Protein, Human (His) is 316 a.a., .

研究背景

醛糖还原酶是一种多功能酶,可催化多种含羰基化合物的 NADPH 依赖性还原,将其转化为相应的醇。值得注意的是,它对各种视网膜异构体表现出强大的酶活性,包括全反式视黄醛、9-顺式视黄醛和13-顺式视黄醛。该酶在来自饮食和脂质的不饱和羰基的解毒中发挥着关键作用,例如巴豆醛、4-羟基壬烯醛、反式-2-己烯醛、反式-2,4-己二烯醛及其谷胱甘肽缀合物羰基(GS-羰基)。值得注意的是,尽管醛糖还原酶具有广泛的底物特异性,但它对葡萄糖不表现出还原酶活性。这种多样化的催化功能凸显了其在细胞解毒过程中的重要性,并强调了其在解决氧化应激相关病症方面的潜在治疗意义。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

O60218 (M1-Y316)

基因 ID

57016

蛋白结构
N-term
6*His
Aldose-reductase (M1-Y316)
Accession # O60218
C-term
同用名
AKR1B10; Aldo-keto reductase family 1 member B10; ARL-1; Aldose reductase-like; Aldose reductase-related protein; ARP; hARP; Small intestine reductase; SI reductase
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Aldose-reductase Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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